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Blood, 1 September 2004, Vol. 104, No. 5, pp. 1324-1326. Prepublished online as a Blood First Edition Paper on May 20, 2004; DOI 10.1182/blood-2004-02-0618. This Article Full Text (PDF) All Versions of this Article: 2004-02-0618v1 104/5/1324    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Clark, A. J Articles by Humbert, P. O Search for Related Content PubMed PubMed Citation Articles by Clark, A. J Articles by Humbert, P. O Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 18, 2004 Accepted April 30, 2004 Cell intrinsic requirement for pRb in erythropoiesis Allison J Clark, Kathryn M Doyle, and Patrick O Humbert* Trescowthick Research Laboratories, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia Trescowthick Research Laboratories, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia; Department of Pathology, University of Melbourne, Parkville, Victoria, Australia * Corresponding author; email: patrick.humbert{at}petermac.org. Rb and family members have been implicated as key regulators of cell proliferation and differentiation. In particular, accumulated data have suggested that pRb is an important controller of erythroid differentiation. However, current published data are conflicting as to whether the role of pRb in erythroid cells is cell intrinsic or non-cell intrinsic. Here, we have made use of an in vitro erythroid differentiation culture system to determine the cell intrinsic requirement for pRb in erythroid differentiation. We demonstrate that loss of pRb function in primary differentiating erythroid cells results in impaired cell cycle exit and terminal differentiation. Furthermore, we have used co-culture experiments to establish that this requirement is cell intrinsic. Together these data unequivocally demonstrate that pRb is required in a cell intrinsic manner for erythroid differentiation and provide clarification as to its role in erythropoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. R. Tallack, J. R. Keys, P. O. Humbert, and A. C. Perkins EKLF/KLF1 Controls Cell Cycle Entry via Direct Regulation of E2f2 J. Biol. Chem., July 31, 2009; 284(31): 20966 - 20974. [Abstract] [Full Text] [PDF] A. M. Pilon, M. O. Arcasoy, H. K. Dressman, S. E. Vayda, Y. 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