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Blood, 15 August 2004, Vol. 104, No. 4, pp. 1217-1223. Prepublished online as a Blood First Edition Paper on April 20, 2004; DOI 10.1182/blood-2004-02-0655. This Article Full Text (PDF) All Versions of this Article: 2004-02-0655v1 104/4/1217    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Arbab, A. S Articles by Frank, J. A Search for Related Content PubMed PubMed Citation Articles by Arbab, A. S Articles by Frank, J. A Related Collections Related Article in Blood Online Related Letter in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 23, 2004 Accepted April 9, 2004 Efficient Magnetic Cell Labeling with Protamine Sulfate Complexed to Ferumoxides for Cellular MRI Ali S Arbab*, Gene T Yocum, Heather Kalish, Elaine K Jordan, Stasia A Anderson, Aarif Y Khakoo, Elizabeth J Read, and Joseph A Frank Laboratory of Diagnostic Radiology Research, National Institutes of Health, Bethesda, Maryland, USA NINDS, National Institutes of Health, Bethesda, Maryland, USA NHLBI, National Institutes of Health, Bethesda, Maryland, USA Department of Transfusion Medicine, National Institutes of Health, Bethesda, Maryland, USA * Corresponding author; email: saali{at}cc.nih.gov. Recently, there have been several reports using various superparamagnetic iron oxide (SPIO) nanoparticles to label mammalian cells for monitoring their temporal and spatial migration in vivo by magnetic resonance imaging (MRI). The purpose of this study was to evaluate the efficiency and toxicity of labeling cells using two commercially available FDA-approved agents, ferumoxides, a suspension of dextran-coated SPIO used as an MRI contrast agent, and protamine sulfate, conventionally used to reverse heparin anticoagulation but also used ex vivo as a cationic transfection agent. After labeling of human mesenchymal (MSC) and hematopoietic (CD 34+) stem cells and other mammalian cells with ferumoxides-protamine sulfate complexes (FE-Pro), cellular toxicity, functional capacity and quantitative cellular iron incorporation were determined. FE-Pro labeled cells demonstrated no short or long-term toxicity, changes in differentiation capacity of the stem cells, or changes in phenotype, when compared to unlabeled cells. Efficient labeling with FE-Pro was observed with iron content per cell varying between 2.01 ± 0.1 picograms for CD 34+ cells and 10.94 ± 1.86 picograms for MSC with 100% of cells labeled. Cell labeling using these agents should facilitate the translation of this method to clinical trials for evaluation of trafficking of infused or transplanted cells by MRI. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: MR-based cellular imaging: ready for prime time? Terence P. F. Gade and Jason A. Koutcher Blood 2004 104: 916-917. [Full Text] [PDF] Related Letter in Blood Online: Chondrogenic differentiation of mesenchymal stem cells is inhibited after magnetic labeling with ferumoxides Jeff W. M. Bulte, Dara L. Kraitchman, Alastair M. Mackay, Mark F. Pittenger, Ali S. Arbab, Gene T. Yocum, Heather Kalish, Elaine K. Jordan, Stasia A. Anderson, Aarif Y. Khakoo, Elizabeth J. Read, and Joseph A. Frank Blood 2004 104: 3410-3413. [Full Text] [PDF] This article has been cited by other articles: Z.-Y. Liu, Y. Wang, C.-H. Liang, X.-H. Li, G.-Y. Wang, H.-J. Liu, and Y. Li In Vitro Labeling of Mesenchymal Stem Cells with Superparamagnetic Iron Oxide by Means of Microbubble-enhanced US Exposure: Initial Experience Radiology, October 1, 2009; 253(1): 153 - 159. [Abstract] [Full Text] [PDF] J. W. M. Bulte In Vivo MRI Cell Tracking: Clinical Studies Am. J. Roentgenol., August 1, 2009; 193(2): 314 - 325. [Abstract] [Full Text] [PDF] Y. L. Wu, Q. Ye, K. Sato, L. M. Foley, T. K. Hitchens, and C. Ho Noninvasive Evaluation of Cardiac Allograft Rejection by Cellular and Functional Cardiac Magnetic Resonance J. Am. Coll. Cardiol. Img., June 1, 2009; 2(6): 731 - 741. [Abstract] [Full Text] [PDF] F. Wang, J. E. Dennis, A. Awadallah, L. A. Solchaga, J. 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Chondrogenic differentiation of mesenchymal stem cells is inhibited after magnetic labeling with ferumoxides Blood, November 15, 2004; 104(10): 3410 - 3413. [Full Text] [PDF]

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