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Blood, 1 June 2005, Vol. 105, No. 11, pp. 4282-4289. Prepublished online as a Blood First Edition Paper on February 17, 2005; DOI 10.1182/blood-2004-02-0756. This Article Full Text (PDF) All Versions of this Article: 2004-02-0756v1 105/11/4282    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jamur, M. C Articles by Oliver, C. Search for Related Content PubMed PubMed Citation Articles by Jamur, M. C Articles by Oliver, C. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 27, 2004 Accepted January 12, 2005 Identification and characterization of undifferentiated mast cells in mouse bone marrow Maria C Jamur, Ana Cristina G Grodzki, Elsa H Berenstein, Majed M Hamawy, Reuben P Siraganian, and Constance Oliver* Departamento de Biologia Celular e Molecular e Bioagentes Patogenicos, Faculdade de Medicina de Ribeirao Preto - Universidade de Sao Pauol, Ribeirao Preto, Brazil Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA * Corresponding author; email: coliver{at}rbp.fmrp.usp.br. Sequential immunomagnetic isolation with two mast cell specific antibodies was used to purify and characterize an undifferentiated mast cell in adult mouse bone marrow that had not been previously recognized. This cell represents 0.02% of the cells in the bone marrow, is CD34+, CD13+, and c-kit+, and does not express FcRI. However, by PCR the cell contains message for the and subunits of FcRI, mast cell specific proteases and carboxypeptidase A. Morphologically, this cell has a large nucleus, little cytoplasm, few cytoplasmic organelles and no cytoplasmic granules. In vitro, in the presence of IL-3 and SCF these cells differentiate only into a granulated mast cell that now expresses CD13, c-kit, mast cell specific gangliosides, FcRI and binds IgE. When injected into lethally irradiated mice, these cells are able to reconstitute the mast cell population in the spleen. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. Cohen, A. Torres, H.-T. Ma, D. Holowka, and B. Baird Ca2+ Waves Initiate Antigen-Stimulated Ca2+ Responses in Mast Cells J. Immunol., November 15, 2009; 183(10): 6478 - 6488. [Abstract] [Full Text] [PDF] M. Watanabe, T. Satoh, Y. Yamamoto, Y. Kanai, H. Karasuyama, and H. Yokozeki Overproduction of IgE Induces Macrophage-Derived Chemokine (CCL22) Secretion from Basophils J. Immunol., October 15, 2008; 181(8): 5653 - 5659. [Abstract] [Full Text] [PDF] J.-i. Kashiwakura, W. Xiao, J. Kitaura, Y. Kawakami, M. Maeda-Yamamoto, J. R. Pfeiffer, B. S. Wilson, U. Blank, and T. Kawakami Pivotal Advance: IgE accelerates in vitro development of mast cells and modifies their phenotype J. Leukoc. Biol., August 1, 2008; 84(2): 357 - 367. [Abstract] [Full Text] [PDF] C. L. Weller, S. J. Collington, A. Hartnell, D. M. Conroy, T. Kaise, J. E. Barker, M. S. Wilson, G. W. Taylor, P. J. Jose, and T. J. Williams Chemotactic action of prostaglandin E2 on mouse mast cells acting via the PGE2 receptor 3 PNAS, July 10, 2007; 104(28): 11712 - 11717. [Abstract] [Full Text] [PDF] C. Waskow, S. Bartels, S. M. Schlenner, C. Costa, and H.-R. Rodewald Kit is essential for PMA-inflammation-induced mast-cell accumulation in the skin Blood, June 15, 2007; 109(12): 5363 - 5370. [Abstract] [Full Text] [PDF] C. Oliver, A. Fujimura, A. M. M. Silveira e Souza, R. Orlandini de Castro, R. P. Siraganian, and M. C. Jamur Mast Cell-specific Gangliosides and Fc{varepsilon}RI Follow the Same Endocytic Pathway From Lipid Rafts in RBL-2H3 Cells J. Histochem. Cytochem., April 1, 2007; 55(4): 315 - 325. [Abstract] [Full Text] [PDF] S. Meindl, U. Schmidt, C. Vaculik, and A. Elbe-Burger Characterization, isolation, and differentiation of murine skin cells expressing hematopoietic stem cell markers J. Leukoc. Biol., October 1, 2006; 80(4): 816 - 826. [Abstract] [Full Text] [PDF] Y. Arinobu, H. Iwasaki, M. F. Gurish, S.-i. Mizuno, H. Shigematsu, H. Ozawa, D. G. Tenen, K. F. Austen, and K. Akashi Developmental checkpoints of the basophil/mast cell lineages in adult murine hematopoiesis PNAS, December 13, 2005; 102(50): 18105 - 18110. [Abstract] [Full Text] [PDF] C. L. Weller, S. J. Collington, J. K. Brown, H. R.P. Miller, A. Al-Kashi, P. Clark, P. J. Jose, A. Hartnell, and T. J. Williams Leukotriene B4, an activation product of mast cells, is a chemoattractant for their progenitors J. Exp. Med., June 20, 2005; 201(12): 1961 - 1971. [Abstract] [Full Text] [PDF]

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