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Blood, 15 November 2004, Vol. 104, No. 10, pp. 3153-3160. Prepublished online as a Blood First Edition Paper on July 29, 2004; DOI 10.1182/blood-2004-03-0809. This Article Full Text (PDF) All Versions of this Article: 2004-03-0809v1 104/10/3153    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Menier, C. Articles by Rouas-Freiss, N. Search for Related Content PubMed PubMed Citation Articles by Menier, C. Articles by Rouas-Freiss, N. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 5, 2004 Accepted July 18, 2004 Erythroblasts secrete the non classical HLA-G molecule from primitive to definitive hematopoiesis Catherine Menier, Michele Rabreau, Jean-Claude Challier, Magali Le Discorde, Edgardo D Carosella, and Nathalie Rouas-Freiss* Service de Recherches en Hemato-Immunologie, Hopital de Saint Louis, Paris, France Institut d'Histo-Cyto-Pathologie, Laboratoire de Foetopathologie, Bordeaux, France Physiopathologie de l'Implantation et du Developpement, Universite Pierre et Marie Curie, Paris, France * Corresponding author; email: freiss{at}dsvidf.cea.fr. The initial steps of primitive hematopoiesis and endothelial vascular formation in the human embryo remain to be defined. Here we report the identification of a novel marker, namely the non classical HLA-G class I molecule which targets both primitive erythroid cells of the yolk sac and endothelial cells from developing vessels. Moreover, HLA-G was present in its soluble form in the erythropoietic lineage in all organs sustaining primitive to definitive erythropoiesis (i.e., aorta-gonad-mesonephros, liver, spleen and bone marrow). The alternatively spliced transcript coding the soluble HLA-G5 molecule was detected in erythroid cells. The corresponding intron 4-retaining 37 kilodalton-HLA-G5 isoform was secreted from the erythroid progenitor stage to the reticulocyte, but was lost in mature erythrocytes and in endothelial cells from differentiated vessels. This study constitutes the first description of an HLA class I antigen expression on the primitive erythroid lineage and provides a way of seeking out both primitive and definitive erythropoiesis using HLA-G5. This new marker, previously known by its immunotolerogeneic properties, may be involved in erythroid differentiation and/or angiogenesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Flajollet, I. Poras, E. D. Carosella, and P. Moreau RREB-1 Is a Transcriptional Repressor of HLA-G J. Immunol., December 1, 2009; 183(11): 6948 - 6959. [Abstract] [Full Text] [PDF] R. Bahri, A. Naji, C. Menier, B. Charpentier, E. D. Carosella, N. Rouas-Freiss, and A. Durrbach Dendritic Cells Secrete the Immunosuppressive HLA-G Molecule upon CTLA4-Ig Treatment: Implication in Human Renal Transplant Acceptance J. Immunol., December 1, 2009; 183(11): 7054 - 7062. [Abstract] [Full Text] [PDF] I. J. WASTOWSKI, P. D. SAMPAIO-BARROS, E. M.I. AMSTALDEN, G. M. PALOMINO, J. F. MARQUES-NETO, J. C.O. CRISPIM, A. C. BIRAL, D. M. RASSI, E. D. CAROSELLA, P. MOREAU, et al. 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Krawice-Radanne, A. Durrbach, C. Guettier, E. D. Carosella, and N. Rouas-Freiss Evidence to Support the Role of HLA-G5 in Allograft Acceptance through Induction of Immunosuppressive/ Regulatory T Cells. J. Immunol., March 1, 2006; 176(5): 3266 - 3276. [Abstract] [Full Text] [PDF] Y. Q. Yao, D. H. Barlow, and I. L. Sargent Differential Expression of Alternatively Spliced Transcripts of HLA-G in Human Preimplantation Embryos and Inner Cell Masses J. Immunol., December 15, 2005; 175(12): 8379 - 8385. [Abstract] [Full Text] [PDF] A. Blaschitz, H. Juch, A. Volz, H. Hutter, C. Daxboeck, G. Desoye, and G. Dohr The soluble pool of HLA-G produced by human trophoblasts does not include detectable levels of the intron 4-containing HLA-G5 and HLA-G6 isoforms Mol. Hum. Reprod., October 1, 2005; 11(10): 699 - 710. [Abstract] [Full Text] [PDF] J. LeMaoult, N. Rouas-Freiss, and E. D. Carosella HLA-G5 expression by trophoblast cells: the facts Mol. Hum. Reprod., October 1, 2005; 11(10): 719 - 722. 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