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Blood, 1 October 2004, Vol. 104, No. 7, pp. 2178-2180. Prepublished online as a Blood First Edition Paper on June 3, 2004; DOI 10.1182/blood-2004-03-0829. This Article Full Text (PDF) All Versions of this Article: 2004-03-0829v1 104/7/2178    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yamaji, S. Articles by Srai, S. K Search for Related Content PubMed PubMed Citation Articles by Yamaji, S. Articles by Srai, S. K Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 5, 2004 Accepted May 7, 2004 Inhibition of iron transport across human intestinal epithelial cells by hepcidin Sachie Yamaji, Paul Sharp, Bala Ramesh, and Surjit K Srai* Biochemistry & Molecular Biology, Royal Free & University College Medical School, UCL, Hampstead, London, United Kingdom Centre for Nutrition & Foos Safety, School of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey, United Kingdom * Corresponding author; email: ksrai{at}rfc.ucl.ac.uk. We have investigated the effects of the iron regulatory peptide hepcidin on iron transport by the human intestinal epithelial Caco-2 cell line. Caco-2 cells were exposed to hepcidin for 24 hours prior to the measurement of both iron transport and transporter protein and mRNA expression. Incubation with hepcidin significantly decreased apical iron uptake by Caco-2 cells. This was accompanied by a decrease in both DMT1(+IRE) protein and mRNA expression. In contrast, iron efflux and IREG1 expression were unaffected by hepcidin. Hepcidin can interact directly with intestinal epithelia. The primary effect of this regulatory peptide is to modulate the apical membrane uptake machinery thereby controlling the amount of iron absorbed from the diet. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Hemojuvelin and hepcidin: the keys to JH? Eric Nisbet-Brown Blood 2004 104: 1918-1919. [Full Text] [PDF] This article has been cited by other articles: M. B Zimmermann, B. Troesch, R. Biebinger, I. Egli, C. Zeder, and R. F Hurrell Plasma hepcidin is a modest predictor of dietary iron bioavailability in humans, whereas oral iron loading, measured by stable-isotope appearance curves, increases plasma hepcidin Am. J. Clinical Nutrition, November 1, 2009; 90(5): 1280 - 1287. [Abstract] [Full Text] [PDF] F. B. Sow, G. R. Alvarez, R. P. Gross, A. R. Satoskar, L. S. Schlesinger, B. S. Zwilling, and W. P. Lafuse Role of STAT1, NF-{kappa}B, and C/EBP{beta} in the macrophage transcriptional regulation of hepcidin by mycobacterial infection and IFN-{gamma} J. Leukoc. Biol., November 1, 2009; 86(5): 1247 - 1258. [Abstract] [Full Text] [PDF] B. Chung, T. Chaston, J. Marks, S. K. Srai, and P. A. Sharp Hepcidin Decreases Iron Transporter Expression in Vivo in Mouse Duodenum and Spleen and in Vitro in THP-1 Macrophages and Intestinal Caco-2 Cells J. Nutr., August 1, 2009; 139(8): 1457 - 1462. [Abstract] [Full Text] [PDF] G. Peslova, J. Petrak, K. Kuzelova, I. Hrdy, P. Halada, P. W. Kuchel, S. Soe-Lin, P. Ponka, R. Sutak, E. Becker, et al. Hepcidin, the hormone of iron metabolism, is bound specifically to {alpha}-2-macroglobulin in blood Blood, June 11, 2009; 113(24): 6225 - 6236. [Abstract] [Full Text] [PDF] M. A Roe, R. Collings, J. R Dainty, D. W Swinkels, and S. J Fairweather-Tait Plasma hepcidin concentrations significantly predict interindividual variation in iron absorption in healthy men Am. J. Clinical Nutrition, April 1, 2009; 89(4): 1088 - 1091. [Abstract] [Full Text] [PDF] K. E. Van Zandt, F. B. Sow, W. C. Florence, B. S. Zwilling, A. R. Satoskar, L. S. Schlesinger, and W. P. Lafuse The iron export protein ferroportin 1 is differentially expressed in mouse macrophage populations and is present in the mycobacterial-containing phagosome J. Leukoc. Biol., September 1, 2008; 84(3): 689 - 700. [Abstract] [Full Text] [PDF] T Chaston, B Chung, M Mascarenhas, J Marks, B Patel, S K Srai, and P Sharp Evidence for differential effects of hepcidin in macrophages and intestinal epithelial cells Gut, March 1, 2008; 57(3): 374 - 382. [Abstract] [Full Text] [PDF] N. P. Mena, A. Esparza, V. Tapia, P. Valdes, and M. T. Nunez Hepcidin inhibits apical iron uptake in intestinal cells Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G192 - G198. [Abstract] [Full Text] [PDF] M. D. Fleming The Regulation of Hepcidin and Its Effects on Systemic and Cellular Iron Metabolism Hematology, January 1, 2008; 2008(1): 151 - 158. [Abstract] [Full Text] [PDF] B. Chung, P. Matak, A. T. McKie, and P. Sharp Leptin Increases the Expression of the Iron Regulatory Hormone Hepcidin in HuH7 Human Hepatoma Cells J. Nutr., November 1, 2007; 137(11): 2366 - 2370. [Abstract] [Full Text] [PDF] F. B. Sow, W. C. Florence, A. R. Satoskar, L. S. Schlesinger, B. S. Zwilling, and W. P. Lafuse Expression and localization of hepcidin in macrophages: a role in host defense against tuberculosis J. Leukoc. Biol., October 1, 2007; 82(4): 934 - 945. [Abstract] [Full Text] [PDF] D. D. Harrison-Findik, D. Schafer, E. Klein, N. A. Timchenko, H. Kulaksiz, D. Clemens, E. Fein, B. Andriopoulos, K. Pantopoulos, and J. Gollan Alcohol Metabolism-mediated Oxidative Stress Down-regulates Hepcidin Transcription and Leads to Increased Duodenal Iron Transporter Expression J. Biol. Chem., August 11, 2006; 281(32): 22974 - 22982. [Abstract] [Full Text] [PDF] H. Chen, G. Huang, T. Su, H. Gao, Z. K. Attieh, A. T. McKie, G. J. Anderson, and C. D. Vulpe Decreased Hephaestin Activity in the Intestine of Copper-Deficient Mice Causes Systemic Iron Deficiency J. Nutr., May 1, 2006; 136(5): 1236 - 1241. [Abstract] [Full Text] [PDF] P S Leung, S K Srai, M Mascarenhas, L J Churchill, and E S Debnam Increased duodenal iron uptake and transfer in a rat model of chronic hypoxia is accompanied by reduced hepcidin expression Gut, October 1, 2005; 54(10): 1391 - 1395. [Abstract] [Full Text] [PDF] S. Rivera, E. Nemeth, V. Gabayan, M. A. Lopez, D. Farshidi, and T. Ganz Synthetic hepcidin causes rapid dose-dependent hypoferremia and is concentrated in ferroportin-containing organs Blood, September 15, 2005; 106(6): 2196 - 2199. [Abstract] [Full Text] [PDF] L. Viatte, J.-C. Lesbordes-Brion, D.-Q. Lou, M. Bennoun, G. Nicolas, A. Kahn, F. Canonne-Hergaux, and S. Vaulont Deregulation of proteins involved in iron metabolism in hepcidin-deficient mice Blood, June 15, 2005; 105(12): 4861 - 4864. [Abstract] [Full Text] [PDF] H. Kawabata, R. E. Fleming, D. Gui, S. Y. Moon, T. Saitoh, J. O'Kelly, Y. Umehara, Y. Wano, J. W. Said, and H. P. Koeffler Expression of hepcidin is down-regulated in TfR2 mutant mice manifesting a phenotype of hereditary hemochromatosis Blood, January 1, 2005; 105(1): 376 - 381. [Abstract] [Full Text] [PDF]

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