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Blood, 1 January 2005, Vol. 105, No. 1, pp. 85-94. Prepublished online as a Blood First Edition Paper on September 9, 2004; DOI 10.1182/blood-2004-03-1002. This Article Full Text (PDF) Supplemental Table and Figures All Versions of this Article: 2004-03-1002v1 105/1/85    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Leberbauer, C. Articles by Mullner, E. W Search for Related Content PubMed PubMed Citation Articles by Leberbauer, C. Articles by Mullner, E. W Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 18, 2004 Accepted August 5, 2004 Different steroids co-regulate long-term expansion versus terminal differentiation in primary human erythroid progenitors Cornelia Leberbauer, Florence Boulme, Gertrud Unfried, Johannes Huber, Hartmut Beug, and Ernst W Mullner* Department of Medical Biochemistry, Division of Molecular Biology, Medical University of Vienna, Max F. Perutz Laboratories - University Departments at the Vienna Biocenter, Vienna, Austria Department of Gynecological Endocrinology, Medical University of Vienna, Vienna, Austria Institute of Molecular Pathology, Vienna, Austria * Corresponding author; email: ernst.muellner{at}univie.ac.at. Outgrowth, long term self renewal and terminal maturation of human erythroid progenitors derived form umbilical cord blood in serum-free medium can be modulated by steroid hormones. Homogenous erythroid cultures, as characterized by flow cytometry and dependence on a specific mixture of physiological proliferation factors were obtained within 8 days from a starting population of mature and immature mononuclear cells. Due to previous results in mouse and chicken erythroblasts, the proliferation-promoting effect of glucocorticoids was not unexpected. Surprisingly, however, androgen had a positive effect on the sustained expansion of human female but not male erythroid progenitors. Under optimal conditions, sustained proliferation of erythroid progenitors resulted in a >109-fold expansion within 60 days. Terminal erythroid maturation was significantly improved by adding human serum and thyroid hormone (T3) to the differentiation medium. This resulted in highly synchronous differentiation of the cells towards enucleated erythrocytes within 6 days, accompanied by massive size decrease and hemoglobin accumulation to levels comparable to those in peripheral blood erythrocytes. Thus, obviously, different, ligand-activated nuclear hormone receptors massively influence the decision between self renewal and terminal maturation in the human erythroid compartment. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S.-J. Lu, Q. Feng, J. S. Park, L. Vida, B.-S. Lee, M. Strausbauch, P. J. Wettstein, G. R. Honig, and R. Lanza Biologic properties and enucleation of red blood cells from human embryonic stem cells Blood, December 1, 2008; 112(12): 4475 - 4484. [Abstract] [Full Text] [PDF] I. S. Naarmann, C. Harnisch, N. Flach, E. Kremmer, H. Kuhn, D. H. Ostareck, and A. Ostareck-Lederer mRNA Silencing in Human Erythroid Cell Maturation: HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN K CONTROLS THE EXPRESSION OF ITS REGULATOR c-Src J. Biol. Chem., June 27, 2008; 283(26): 18461 - 18472. [Abstract] [Full Text] [PDF] L. Ferrucci, M. Maggio, S. Bandinelli, S. Basaria, F. Lauretani, A. Ble, G. Valenti, W. B. Ershler, J. M. Guralnik, and D. L. Longo Low testosterone levels and the risk of anemia in older men and women. Arch Intern Med, July 10, 2006; 166(13): 1380 - 1388. [Abstract] [Full Text] [PDF]

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