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Blood, 1 November 2004, Vol. 104, No. 9, pp. 2801-2809. Prepublished online as a Blood First Edition Paper on July 15, 2004; DOI 10.1182/blood-2004-03-1193. This Article Full Text (PDF) Supplemental Videos All Versions of this Article: 2004-03-1193v1 104/9/2801    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gunzer, M. Articles by Grabbe, S. Search for Related Content PubMed PubMed Citation Articles by Gunzer, M. Articles by Grabbe, S. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 30, 2004 Accepted June 4, 2004 A spectrum of biophysical interaction modes between T cells and different antigen presenting cells during priming in 3-D collagen and in vivo Matthias Gunzer*, Carsten Weishaupt, Anja Hillmer, Yasmin Basoglu, Peter Friedl, Kurt E Dittmar, Waldemar Kolanus, Georg Varga, and Stephan Grabbe Junior Research Group Immunodynamics, German Research Centre for Biotechnology, Braunschweig, Germany; Department of Dermatology, University of Muenster, Muenster, Germany Department of Dermatology, University of Muenster, Muenster, Germany Junior Research Group Immunodynamics, German Research Centre for Biotechnology, Braunschweig, Germany Department of Dermatology, University of Wuerzburg, Wuerzburg, Germany Institute for Molecular Physiology and Developmental Biology, University of Bonn, Bonn, Germany * Corresponding author; email: mgunzer{at}gbf.de. For activation T cells engage antigen presenting cells (APC) in lymphatic tissues. The contact duration and kinetics (static versus dynamic) vary considerably in different model systems, however, it is unclear, whether T cells, APC or the environment are responsible for the observed discrepancies. Using 3-D collagen matrices as structural scaffold, we directly compared the kinetics of T cell engagement and activation by functionally major APC types, i.e. dendritic cells (DC) and resting or activated B cells. Resting B cells engaged T cells in long-lived (several hours), adhesive, and LFA-1 dependent conjugates in 3-D collagen as well as in intact lymph nodes in vivo. DC and pre-activated B cells, however, supported predominantly dynamic, short-lived (minutes) and sequential contacts to T cells which were dependent on high cytoskeletal activity of the APC, but could not be inhibited by anti-LFA-1 treatment. Naive T cells were most strongly activated by DC and activated B cells, while resting B cells were 100 fold less efficient to induce T cell proliferation. Thus, in the same 3-D environment, naive T cells respond with a spectrum of different interaction modes dependent on the type and activation state of the APC. Thereby, more dynamic interaction kinetics are positively correlated with higher T cell priming efficiency. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: New ways for lymphocytes to meet Michael L. Dustin Blood 2004 104: 2618-2619. [Full Text] [PDF] This article has been cited by other articles: C. J. Kroger, S. Amoah, and M. A. Alexander-Miller Cutting Edge: Dendritic Cells Prime a High Avidity CTL Response Independent of the Level of Presented Antigen J. Immunol., May 1, 2008; 180(9): 5784 - 5788. [Abstract] [Full Text] [PDF] S. Balkow, F. Krux, K. Loser, J. U. Becker, S. Grabbe, and U. 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