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Blood, 15 September 2004, Vol. 104, No. 6, pp. 1688-1695. Prepublished online as a Blood First Edition Paper on June 3, 2004; DOI 10.1182/blood-2004-04-1247. This Article Full Text (PDF) All Versions of this Article: 2004-04-1247v1 104/6/1688    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Waskow, C. Articles by Rodewald, H.-R. Search for Related Content PubMed PubMed Citation Articles by Waskow, C. Articles by Rodewald, H.-R. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 9, 2004 Accepted May 12, 2004 Rescue of lethal c-KitW/W mice by erythropoietin Claudia Waskow, Grzegorz Terszowski, Celine Costa, Max Gassmann, and Hans-Reimer Rodewald* Immunology, University of Ulm, Ulm, Germany Veterinary Physiology, University of Zuerich, Zuerich, Switzerland * Corresponding author; email: hans-reimer.rodewald{at}medizin.uni-ulm.de. Homozygous natural "white-spotted" (W) mutations in the gene encoding the receptor tyrosine kinase c-Kit are associated with hypoplastic bone marrow, severe macrocytic anemia and lethality during early postnatal life. C-KitW/W mice can be rescued by wild type hematopoietic stem cells (HSC) but it is not known whether the lethality of c-KitW/W mice is due to HSC failure or due to defects specific for erythropoiesis. Here we show that transgenic expression of erythropoietin (Epo) can overcome the lethality caused by the c-KitW/W mutation. In W-mutant mice rescued by Epo, termed "Wepo", colony forming units erythrocytes (CFU-E) are rescued to normal frequencies. Hence, Epo receptor signals can partially bypass the strict requirement for c-Kit signaling in erythropoiesis in the absence of c-Kit in vivo. Using a series of W and rescue mouse strains, we define here the erythropoietic threshold permitting survival in vivo. The lethality of c-KitW/W mice has precluded analyses of this crucial receptor-ligand pair in adult stem/progenitor cells. Our strategy to generate viable c-KitW/W mice will be useful to analyze the role of this important receptor tyrosine kinase in adult life in vivo. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. A. Schneider, S. M. Schlenner, T. B. Feyerabend, M. Wunderlin, and H.-R. Rodewald Molecular mechanism of mast cell mediated innate defense against endothelin and snake venom sarafotoxin J. Exp. Med., October 29, 2007; 204(11): 2629 - 2639. [Abstract] [Full Text] [PDF] C. Waskow, S. Bartels, S. M. Schlenner, C. Costa, and H.-R. Rodewald Kit is essential for PMA-inflammation-induced mast-cell accumulation in the skin Blood, June 15, 2007; 109(12): 5363 - 5370. [Abstract] [Full Text] [PDF] M. Moniruzzaman, K. Sakamaki, Y. Akazawa, and T. Miyano Oocyte growth and follicular development in KIT-deficient Fas-knockout mice Reproduction, January 1, 2007; 133(1): 117 - 125. [Abstract] [Full Text] [PDF] K. Heinicke, O. Baum, O. O. Ogunshola, J. Vogel, T. Stallmach, D. P. Wolfer, S. Keller, K. Weber, P. D. Wagner, M. Gassmann, et al. Excessive erythrocytosis in adult mice overexpressing erythropoietin leads to hepatic, renal, neuronal, and muscular degeneration Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2006; 291(4): R947 - R956. [Abstract] [Full Text] [PDF] A. Bashamboo, A. H. Taylor, K. Samuel, J.-J. Panthier, A. D. Whetton, and L. M. Forrester The survival of differentiating embryonic stem cells is dependent on the SCF-KIT pathway J. Cell Sci., August 1, 2006; 119(15): 3039 - 3046. [Abstract] [Full Text] [PDF] M. Wouters, K. Smans, and J.-M. Vanderwinden WZsGreen/+: a new green fluorescent protein knock-in mouse model for the study of KIT-expressing cells in gut and cerebellum Physiol Genomics, August 11, 2005; 22(3): 412 - 421. [Abstract] [Full Text] [PDF] A. L. Smith, F. M. Ellison, J. P. McCoy Jr., and J. Chen c-Kit Expression and Stem Cell Factor-Induced Hematopoietic Cell Proliferation Are Up-Regulated in Aged B6D2F1 Mice J. Gerontol. A Biol. Sci. Med. Sci., April 1, 2005; 60(4): 448 - 456. [Abstract] [Full Text] [PDF] G. Terszowski, C. Waskow, P. Conradt, D. Lenze, J. Koenigsmann, D. Carstanjen, I. Horak, and H.-R. Rodewald Prospective isolation and global gene expression analysis of the erythrocyte colony-forming unit (CFU-E) Blood, March 1, 2005; 105(5): 1937 - 1945. [Abstract] [Full Text] [PDF]

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