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Blood, 1 January 2005, Vol. 105, No. 1, pp. 111-114. Prepublished online as a Blood First Edition Paper on July 1, 2004; DOI 10.1182/blood-2004-04-1306. This Article Full Text (PDF) Supplemental Figure All Versions of this Article: 2004-04-1306v1 105/1/111    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhang, W. J Articles by Choi, K. Search for Related Content PubMed PubMed Citation Articles by Zhang, W. J Articles by Choi, K. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 9, 2004 Accepted June 7, 2004 Modulation of hematopoietic and endothelial cell differentiation from mouse embryonic stem cells by different culture conditions Wen J Zhang, Changwon Park, Elizabeth Arentson, and Kyunghee Choi* Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA; Developmental Biology Program, Washington University School of Medicine, St. Louis, MO, USA * Corresponding author; email: kchoi{at}pathology.wustl.edu. Embryonic stem (ES) cells can differentiate into many different somatic cells in culture. To better correlate hematopoietic and endothelial cell differentiation of ES cells in currently available protocols, we compared Flk-1, Scl, VE-cadherin, CD45, or Ter-119 expressing cells generated in embryoid bodies (EBs) and on OP9 cells. We report that the kinetics of Scl and Flk-1 expression were similar in EBs and OP9 cells, although Flk-1 expression was extended on OP9 cells. CD45+ and Ter-119+ cells developed more efficiently in EBs, while VE-cadherin+ cells developed predominantly on OP9 cells. Cell sorting and replating studies showed that Scl+ cells, not Flk-1+ or VE-cadherin+ cells, were enriched for primitive and definitive hematopoietic progenitors. Our studies indicate that optimal hematopoietic and endothelial cell differentiation occur in EBs and on OP9 cells, respectively. Regardless of the culture systems used, Scl is the most relevant marker for enriching primitive and definitive hematopoietic progenitors. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Nishikii, K. Eto, N. Tamura, K. Hattori, B. Heissig, T. Kanaji, A. Sawaguchi, S. Goto, J. Ware, and H. Nakauchi Metalloproteinase regulation improves in vitro generation of efficacious platelets from mouse embryonic stem cells J. Exp. Med., August 4, 2008; 205(8): 1917 - 1927. [Abstract] [Full Text] [PDF] R. C. R. Perlingeiro Endoglin is required for hemangioblast and early hematopoietic development Development, August 15, 2007; 134(16): 3041 - 3048. [Abstract] [Full Text] [PDF] G. Shinoda, K. Umeda, T. Heike, M. Arai, A. Niwa, F. Ma, H. Suemori, H. Y. Luo, D. H. K. Chui, R. Torii, et al. {alpha}4-Integrin+ endothelium derived from primate embryonic stem cells generates primitive and definitive hematopoietic cells Blood, March 15, 2007; 109(6): 2406 - 2415. [Abstract] [Full Text] [PDF] A. L. Olsen, D. L. Stachura, and M. J. Weiss Designer blood: creating hematopoietic lineages from embryonic stem cells Blood, February 15, 2006; 107(4): 1265 - 1275. [Abstract] [Full Text] [PDF]

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