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Blood, 1 January 2005, Vol. 105, No. 1, pp. 31-39. Prepublished online as a Blood First Edition Paper on September 9, 2004; DOI 10.1182/blood-2004-04-1369. This Article Full Text (PDF) Supplemental Table and Videos All Versions of this Article: 2004-04-1369v1 105/1/31    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Liu, C. Articles by Takahama, Y. Search for Related Content PubMed PubMed Citation Articles by Liu, C. Articles by Takahama, Y. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 9, 2004 Accepted August 11, 2004 Role of CCL21 in Recruitment of T Precursor Cells to Fetal Thymus Cunlan Liu, Tomoo Ueno, Sachiyo Kuse, Fumi Saito, Takeshi Nitta, Luca Piali, Hideki Nakano, Terutaka Kakiuchi, Martin Lipp, Georg A Hollander, and Yousuke Takahama* Division of Experimental Immunology, Institute for Genome Research, University of Tokushima, Tokushima, Japan Pediatric Immunology, Department of Research, University of Basel, Basel, Switzerland Department of Immunology, Toho University School of Medicine, Tokyo, Japan Department of Tumor Genetics and Immunogenetics, Max-Delbruck Center for Molecular Medicine, Berlin, Germany * Corresponding author; email: takahama{at}genome.tokushima-u.ac.jp. During embryonic development, T lymphoid precursor cells colonize the thymus. Chemoattraction by the fetal thymus is thought to mediate T precursor cell colonization. However, the molecules that attract T precursor cells to the thymus remain unclear. By devising time-lapse visualization in culture, the present results show that alymphoid fetal thymus lobes attract T precursor cells from fetal liver or fetal blood. CD4-CD8-CD25-CD44+ fetal thymocytes retained the activity to specifically re-enter the thymus. The attraction was predominantly due to I-A-expressing thymic epithelial cells and was mediated by pertussis-toxin-sensitive G-protein signals. Among the chemokines produced by the fetal thymus, CCL21, CCL25, and CXCL12 could attract CD4-CD8-CD25-CD44+ fetal thymocytes. However, fetal thymus colonization was markedly diminished by neutralizing antibodies specific for CCL21 and CCL25, but not affected by anti-CXCL12 antibody. Fetal thymus colonization was partially defective in CCL21-deficient plt/plt mice and was further diminished by anti-CCL25 antibody. These results indicate that CCL21 is involved in the recruitment of T cell precursors to the fetal thymus, and suggest that the combination of CCL21 and CCL25 plays a major role in fetal thymus colonization. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. A. Sultana, S. Tomita, M. Hamada, Y. Iwanaga, Y. Kitahama, N. Van Khang, S. Hirai, I. Ohigashi, S. Nitta, T. Amagai, et al. Gene expression profile of the third pharyngeal pouch reveals role of mesenchymal MafB in embryonic thymus development Blood, March 26, 2009; 113(13): 2976 - 2987. [Abstract] [Full Text] [PDF] M. A. Haniffa, M. P. Collin, C. D. Buckley, and F. Dazzi Mesenchymal stem cells: the fibroblasts' new clothes? Haematologica, February 1, 2009; 94(2): 258 - 263. [Abstract] [Full Text] [PDF] L. T. Jeker, T. Barthlott, M. P. Keller, S. Zuklys, M. Hauri-Hohl, C.-X. Deng, and G. A. Hollander Maintenance of a normal thymic microenvironment and T-cell homeostasis require Smad4-mediated signaling in thymic epithelial cells Blood, November 1, 2008; 112(9): 3688 - 3695. [Abstract] [Full Text] [PDF] P. Zhao, S. G. Waxman, and B. C. Hains Modulation of Thalamic Nociceptive Processing after Spinal Cord Injury through Remote Activation of Thalamic Microglia by Cysteine Cysteine Chemokine Ligand 21 J. Neurosci., August 15, 2007; 27(33): 8893 - 8902. [Abstract] [Full Text] [PDF] M. Itoi, N. Tsukamoto, H. Yoshida, and T. Amagai Mesenchymal cells are required for functional development of thymic epithelial cells Int. Immunol., August 1, 2007; 19(8): 953 - 964. [Abstract] [Full Text] [PDF] K. Mori, M. Itoi, N. Tsukamoto, H. Kubo, and T. Amagai The perivascular space as a path of hematopoietic progenitor cells and mature T cells between the blood circulation and the thymic parenchyma Int. Immunol., June 1, 2007; 19(6): 745 - 753. [Abstract] [Full Text] [PDF] T. S. P. Heng, G. L. Goldberg, D. H. D. Gray, J. S. Sutherland, A. P. Chidgey, and R. L. Boyd Effects of Castration on Thymocyte Development in Two Different Models of Thymic Involution J. Immunol., September 1, 2005; 175(5): 2982 - 2993. [Abstract] [Full Text] [PDF]

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