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Blood, 15 November 2004, Vol. 104, No. 10, pp. 3228-3230.
Prepublished online as a Blood First Edition Paper on July 22, 2004; DOI 10.1182/blood-2004-04-1428.
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Submitted April 16, 2004
Accepted June 17, 2004
Visualization of Lymphatic Vessels Through NF- B Activity
Marcia R Saban, Sylvie Memet, David G Jackson, John Ash, Aurelio A Roig, Alain Israel, and Ricardo Saban*
Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
Unite de Biologie Moleculaire de l'Expression Genique, Institut Pasteur, Paris, France
MRC Human Immunology Unit, The Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford, United Kingdom
Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
* Corresponding author; email: ricardo-saban{at}ouhsc.edu.
The molecular biology of lymphatics is only rudimentary owing to longstanding absence of specific markers and scanty is the information regarding bladder lymphatic vessels. By using mice with a reporter gene for NF- B activity (B -lacZ) in combination with immunohistochemical staining with a specific lymphatic marker (LYVE-1), we show, for the first time, that NF-B is constitutively active in lymphatic endothelium in the urinary bladder, uterus, intestine, heart, and airways. Tie2-lacZ) mice confirmed that the structures observed in B mice were not blood vessels. In addition, acute instillation of LPS or TNF into the B-lacZ) mouse bladder revealed the capacity of this transgenic in reporting inducible NF-B activity. Our findings demonstrate an overriding constitutive NF-B activity in the lymphatic system. They also provide a working model for detecting lymphatic vessels, and evoke testable hypotheses regarding the role of lymphatic vessels in health and disease.
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