SEARCH:   Advanced

Blood, 1 February 2005, Vol. 105, No. 3, pp. 1010-1015. Prepublished online as a Blood First Edition Paper on September 21, 2004; DOI 10.1182/blood-2004-04-1498. This Article Full Text (PDF) All Versions of this Article: 2004-04-1498v1 105/3/1010    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Laukkanen, M. O Articles by Dunbar, C. E Search for Related Content PubMed PubMed Citation Articles by Laukkanen, M. O Articles by Dunbar, C. E Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 26, 2004 Accepted September 13, 2004 Low dose total body irradiation causes clonal fluctuation of primate hematopoietic stem and progenitor cells Mikko O Laukkanen, Ken Kuramoto, Boris Calmels, Masaaki Takatoku, Christof von Kalle, Robert E Donahue, and Cynthia E Dunbar* Molecular Hematopoiesis Section, Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD, USA Division of Experimental Hematology, Children's Research Foundation, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA * Corresponding author; email: Dunbarc{at}nhlbi.nih.gov. Due to high frequency of side effects caused by high dose total body irradiation (TBI) the non-myeloablative regimen together with cytotoxic agents is currently used especially for elderly patients. However, immediate and long-term effects of low dose irradiation employed in allogeneic transplantation on stem cells is less well known. We have studied the effect of low dose 3 Gy TBI on the number of hematopoietic stem cell (HSC) clones contributing simultaneously to granulocyte production in rhesus macaque. The number of clones post-3 Gy TBI decreased markedly by 2-3 weeks post-3 Gy TBI, followed by a period of clonal instability, and recovery to almost pre-3 Gy TBI clonal diversity. The clones accounting for this recovery contributed pre-3 Gy TBI, suggesting the profound initial impact of TBI was on a pool of progenitor cells, where as most of the more primitive HSCs remained unaffected and were able to regain contributing to hematopoiesis after recovery. Clonal fluctuation may indirectly suggest the presence of short term/long term HSC populations in rhesus macaque bone marrow as reported in a mouse model. The results indicate that even low dose irradiation affects hematopoietic clonal dynamics, and have implications for design of conditioning regimens for transplantation purposes. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. R. Bauer Jr, M. Hai, L. M. Tuschong, T. H. Burkholder, Y.-c. Gu, R. A. Sokolic, C. Ferguson, C. E. Dunbar, and D. D. Hickstein Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy Blood, November 15, 2006; 108(10): 3313 - 3320. [Abstract] [Full Text] [PDF] B. Heissig, S. Rafii, H. Akiyama, Y. Ohki, Y. Sato, T. Rafael, Z. Zhu, D. J. Hicklin, K. Okumura, H. Ogawa, et al. Low-dose irradiation promotes tissue revascularization through VEGF release from mast cells and MMP-9-mediated progenitor cell mobilization J. Exp. Med., September 19, 2005; 202(6): 739 - 750. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020