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Blood, 15 November 2004, Vol. 104, No. 10, pp. 3302-3304.
Prepublished online as a Blood First Edition Paper on July 27, 2004; DOI 10.1182/blood-2004-04-1536.
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Submitted April 22, 2004
Accepted July 6, 2004
A comparative analysis of T cell costimulation and CD43 activation reveals novel signaling pathways and target genes
M. Lienhard Schmitz*, Ivan Mattioli, Oliver Dittrich-Breiholz, Michael Kracht, and Mark Livingstone
Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland
Institute of Pharmacology, Medical School Hannover, Hannover, Germany
Cell Signaling Technology, Inc., Beverly, MA, USA
* Corresponding author; email: Lienhard.Schmitz{at}ibc.unibe.ch.
The CD43 lymphocyte surface receptor is involved in the regulation of lymphocyte adhesion and activation. Many CD43 functions remain controversial or unclear and it is not known to which extent CD43 signaling pathways are shared with or distinct from those employed by the TCR. Here we systematically compared signaling events and target gene expression induced by CD43 or T cell costimulation in primary human peripheral T cells. These studies identify NF- B p65 serine 468 as a novel inducible phosphorylation site strongly induced by T cell costimulation and only weakly triggered by CD43 ligation. We also identified CD43 as a novel JNK activator and a comprehensive analysis of further signaling events suggests that both stimuli employ overlapping but also distinct signaling pathways. Microarray analysis of inflammatory genes shows one group of genes co-regulated by both stimuli and two further groups of target genes affected solely by costimulation or primarily by CD43.
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