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Blood, 15 January 2005, Vol. 105, No. 2, pp. 617-626. Prepublished online as a Blood First Edition Paper on September 16, 2004; DOI 10.1182/blood-2004-04-1649. This Article Full Text (PDF) All Versions of this Article: 2004-04-1649v1 105/2/617    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Vodyanik, M. A Articles by Slukvin, I. I Search for Related Content PubMed PubMed Citation Articles by Vodyanik, M. A Articles by Slukvin, I. I Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 29, 2004 Accepted September 8, 2004 Human embryonic stem cell-derived CD34+ cells: efficient production in the co-culture with OP9 stromal cells and analysis of lymphohematopoietic potential Maxim A Vodyanik, Jack A Bork, James A Thomson, and Igor I Slukvin* Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI, USA Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI, USA; WiCell Research Institute, Madison, WI, USA; Department of Anatomy, Department of Obstetrics and Gynecology, University of Wisconsin, Madison, WI, USA Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI, USA; WiCell Research Institute, Madison, WI, USA; Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI, USA * Corresponding author; email: islukvin{at}wisc.edu. Embryonic stem (ES) cells have the potential to serve as an alternative source of hematopoietic precursors for transplantation and for the study of hematopoietic cell development. Using co-culture of human ES (hES) cells with OP9 bone marrow stromal cells, we were able to obtain up to 20% of CD34+ cells and isolate up to 107 CD34+ cells with more than 95% purity from a similar number of initially plated hES cells after 8-9 days of culture. The hES cell-derived CD34+ cells were highly enriched in colony-forming cells, cells expressing hematopoiesis-associated genes GATA-1, GATA-2, SCL, and Flk-1, and retained clonogenic potential after in vitro expansion. CD34+ cells displayed the phenotype of primitive hematopoietic progenitors as defined by co-expression of CD90, CD117, and CD164, along with a lack of CD38 expression, and contained aldehyde dehydrogenase-positive cells as well cells with verapamil-sensitive ability to efflux rhodamine 123. When cultured on MS-5 stromal cells in the presence of SCF, Flt3-L, IL7, and IL3, isolated CD34+ cells differentiated into lymphoid (B and NK cells) as well as myeloid (macrophages and granulocytes) lineages. These data indicate that CD34+ cells generated through hES/OP9 co-culture display several features of definitive hematopoietic stem cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. S. Kaufman Toward clinical therapies using hematopoietic cells derived from human pluripotent stem cells Blood, October 22, 2009; 114(17): 3513 - 3523. [Abstract] [Full Text] [PDF] S. L. McKinney-Freeman, O. Naveiras, F. Yates, S. Loewer, M. Philitas, M. Curran, P. J. Park, and G. Q. Daley Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells Blood, July 9, 2009; 114(2): 268 - 278. [Abstract] [Full Text] [PDF] Y. Yokoyama, T. Suzuki, M. Sakata-Yanagimoto, K. 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