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Blood, 15 December 2004, Vol. 104, No. 13, pp. 3918-3926. Prepublished online as a Blood First Edition Paper on August 26, 2004; DOI 10.1182/blood-2004-05-1845. This Article Full Text (PDF) Supplemental Tables All Versions of this Article: 2004-05-1845v1 104/13/3918    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Haddad, R. Articles by Canque, B. Search for Related Content PubMed PubMed Citation Articles by Haddad, R. Articles by Canque, B. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 14, 2004 Accepted July 28, 2004 Molecular characterization of early human T/NK and B lymphoid progenitor cells in umbilical cord blood Rima Haddad, Philippe Guardiola, Brigitte Izac, Christelle Thibault, Jerry Radich, Anne-Lise Delezoide, Claude Baillou, Francois M Lemoine, Jean Claude Gluckman, Francoise Pflumio, and Bruno Canque* EMI-0013 Institut National de la Sante et de la Recherche Medicale, Universite Paris 7, and Laboratoire d'Immunologie Cellulaire et Immunopathologie de l'Ecole Pratique des Hautes Etudes, Institut Universitaire d'Hematologie, hopital Saint-Louis, Paris, France Experimental Pathology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA Departement d'Hematologie, Institut Cochin, INSERM U567, Paris, France Institut de Genetique et Biologie Moleculaire et Cellulaire, Strasbourg, France Service de Biologie du Developpement, hopital Robert Debre AP-HP, Paris, France CNRS UMR 7087, hopital Pitie-Salpetriere AP-HP, Paris, France * Corresponding author; email: bruno.canque{at}chu-stlouis.fr. Abstract The early stages of human lymphopoiesis are poorly characterized. Here, we compared the lymphoid potential of a novel umbilical cord blood CD34+CD45RAhiCD7+ hematopoietic progenitor cell (HPC) population with that of CD34+CD45RAhiLin-CD10+ HPCs, previously proposed as candidate common lymphoid progenitors. Limiting-dilution and clonal analysis, fetal thymic organ cultures and culture onto Notch ligand Delta-like-1-expressing OP9 cells, showed that although CD34+CD45RAhiCD7+ HPCs could generate cells of the three lymphoid lineages, their potential was skewed toward the T/NK lineages. In contrast, CD34+CD45RAhiLin-CD10+ HPCs predominantly exhibited a B cell potential. Gene expression profiling with DNA micro-arrays confirmed that CD34+CD45RAhiCD7+ HPCs selectively expressed T-lymphoid and NK lineage-committed genes while retaining expression of genes affiliated to the granulo-monocytic lineage, whereas CD34+CD45RAhiLin-CD10+ HPCs displayed a typical pro-B cell transcription profile and essentially lacked genes unrelated to the B lineage. In addition, both populations could be generated in vitro from CD34+CD45RAintCD7- and CD34+CD45RAhiLin- HPCs with mixed lympho-myeloid potential, from which they emerged independently with different growth/differentiation factor requirements. These findings indicate that CD34+CD45RAhiCD7+ and CD34+CD45RAhiLin-CD10+ HPCs correspond to multipotent early lymphoid progenitors polarized toward either the T/NK or B lineage, respectively. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: G. Awong, E. Herer, C. D. Surh, J. E. Dick, R. N. 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