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Blood, 15 February 2005, Vol. 105, No. 4, pp. 1606-1613. Prepublished online as a Blood First Edition Paper on October 26, 2004; DOI 10.1182/blood-2004-06-2406. This Article Full Text (PDF) All Versions of this Article: 2004-06-2406v1 105/4/1606    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Liu, A. Articles by Klein, E. Search for Related Content PubMed PubMed Citation Articles by Liu, A. Articles by Klein, E. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 24, 2004 Accepted October 12, 2004 PSK and Trx80 inhibit B cell growth in EBV infected cord blood mononuclear cells through T cells activated by the monocyte products IL15 and IL12 Anquan Liu*, Jack L Arbiser, Arne Holmgren, George Klein, and Eva Klein Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden Department of Dermatology, Emory University School of Medicine, Atlanta, GA, USA Medical Nobel Institute for Biochemistry, Department of Biophysics and Biochemistry, Karolinska Institute, Stockholm, Sweden * Corresponding author; email: Anquan.Liu{at}mtc.ki.se. EBV specific immunological memory is not transferred from mother to child. In vitro infection of cord blood cells can therefore readily lead to the outgrowth of transformed B lymphocytes. We found that the immunomodulator PSK or the mitogenic cytokine Trx80 inhibited the EBV induced B cell proliferation. Using SAP induction as a sign for T, NK cell activation we could follow it without any need for cell separation because neither macrophages nor B lymphocytes express SAP. The results suggest the following scenario: EBV infected and activated B lymphocytes. They were recognized by T cells that became posed for responding to cytokines produced by monocytes. PSK and Trx80, which is a secreted C-terminally truncated thioredoxin, both activated the monocytes which then produced cytokines in the presence of the primed T cells. PSK induced IL-15 while Trx80 induced IL-12 production. Both cytokines activated the T cells for function. PI 3-kinase and ROS were involved in the PSK induced activation of monocytes. Restimulation of the cultures with EBV transformed B cells generated specific cytotoxic activity. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Liu, H.-E. Claesson, Y. Mahshid, G. Klein, and E. Klein Leukotriene B4 activates T cells that inhibit B-cell proliferation in EBV-infected cord blood-derived mononuclear cell cultures Blood, March 1, 2008; 111(5): 2693 - 2703. [Abstract] [Full Text] [PDF]

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