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Blood, 1 February 2005, Vol. 105, No. 3, pp. 1256-1264. Prepublished online as a Blood First Edition Paper on September 23, 2004; DOI 10.1182/blood-2004-06-2416. This Article Full Text (PDF) All Versions of this Article: 2004-06-2416v1 105/3/1256    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Garnache-Ottou, F. Articles by Saas, P. Search for Related Content PubMed PubMed Citation Articles by Garnache-Ottou, F. Articles by Saas, P. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 28, 2004 Accepted September 15, 2004 Expression of the myeloid associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells Francine Garnache-Ottou, Laurence Chaperot, Sabeha Biichle, Christophe Ferrand, Jean-Paul Remy-Martin, Eric Deconinck, Patrick Darodes de Tailly, Benedicte Bulabois, Jacqueline Poulet, Emilienne Kuhlein, Marie-Christine Jacob, Veronique Salaun, Michel Arock, Bernard Drenou, Francoise Schillinger, Estelle Seilles, Pierre Tiberghien, Jean-Claude Bensa, Joel Plumas, and Philippe Saas* Etablissement Francais du Sang Bourgogne Franche-Comte, Besancon, France; Unite Mixte EFS/Universite EA2284/Inserm U645, IFR133, Besancon, France Etablissement Francais du Sang Rhone-Alpes, Grenoble, France Unite Mixte EFS/Universite EA2284/Inserm U645, IFR133, Besancon, France Unite Mixte EFS/Universite EA2284/Inserm U645, IFR133, Besancon, France; Service d'Hematologie, CHU Jean Minjoz, Besancon, France Etablissement Francais du Sang Bourgogne Franche-Comte, Besancon, France Service d'Immunologie, CHU Rangueil, Toulouse, France Hematologie, CHU Cote de Nacre, Caen, France Cellular and Molecular Hematology, CNRS FRE 2444, Faculty of Pharmacy, Paris, France Laboratoire d'Hematologie, CHU Pontchaillou, Rennes, France * Corresponding author; email: philippe.saas{at}efs.sante.fr. A new entity of acute leukemia co-expressing CD4+CD56+ markers without any other lineage-specific markers has been recently identified as arising from lymphoid-related plasmacytoid dendritic cells (pDC). Cells from a patient with such CD4+CD56+ lineage negative leukemia was unexpectedly found in our laboratory to also express the myeloid marker CD33. To confirm the diagnosis of pDC leukemia despite the CD33 expression, we demonstrated that the leukemic cells indeed exhibited pDC phenotypic and functional properties. In 7 out of 8 other patients with CD4+CD56+ pDC malignancies, we were able to confirm that the tumor cells expressed CD33 although with variable expression levels. CD33 expression was shown by flow cytometry, RT-PCR and immunoblot analysis. Furthermore, CD33 monoclonal antibody (mAb) stimulation of purified CD4+CD56+ leukemic cells led to cytokine secretion thus confirming the presence of a functional CD33 on these leukemic cells. Moreover, we found that circulating pDC in normal subjects also weakly express CD33. Overall, our results demonstrate that the expression of CD33 on CD4+CD56+ lineage negative cells should not exclude the diagnosis of pDC leukemia and underline that pDC specific markers should be used at diagnosis for CD4+CD56+ malignancies. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Angelot, E. Seilles, S. Biichle, Y. Berda, B. Gaugler, J. Plumas, L. Chaperot, F. Dignat-George, P. Tiberghien, P. Saas, et al. Endothelial cell-derived microparticles induce plasmacytoid dendritic cell maturation: potential implications in inflammatory diseases Haematologica, November 1, 2009; 94(11): 1502 - 1512. [Abstract] [Full Text] [PDF] T. Marafioti, J. C. Paterson, E. Ballabio, K. K. Reichard, S. Tedoldi, K. Hollowood, M. Dictor, M.-L. Hansmann, S. A. Pileri, M. J. Dyer, et al. Novel markers of normal and neoplastic human plasmacytoid dendritic cells Blood, April 1, 2008; 111(7): 3778 - 3792. [Abstract] [Full Text] [PDF]

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