SEARCH:   Advanced

Blood, 1 May 2005, Vol. 105, No. 9, pp. 3641-3647. Prepublished online as a Blood First Edition Paper on January 13, 2005; DOI 10.1182/blood-2004-06-2468. This Article Full Text (PDF) All Versions of this Article: 2004-06-2468v1 105/9/3641    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Reid, G. S Articles by Schultz, K. R Search for Related Content PubMed PubMed Citation Articles by Reid, G. S Articles by Schultz, K. R Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 29, 2004 Accepted January 3, 2005 CpG stimulation of precursor B lineage acute lymphoblastic leukemia induces a distinct change in costimulatory molecule expression and shifts allogeneic T cells towards a Th1 response Gregor S Reid*, Kevin She, Luke Terrett, Michael R Food, Jacqueline D Trudeau, and Kirk R Schultz Department of Pediatrics, Division of Hematology/Oncology/Bone Marrow Transplantation, University of British Columbia and BC Children's Hospital, Vancouver, BC, Canada * Corresponding author; email: grogreid{at}interchange.ubc.ca. Immunostimulatory DNA containing unmethylated CpG induces the development of Th1 immune responses. The response of B cells to CpG stimulation involves increased proliferation, cytokine production and costimulatory molecule expression. Similar effects have been observed following CpG stimulation of a variety of malignant B cells. Pediatric precursor-B ALL cells express low levels of costimulatory molecules and are generally poor stimulators of T cell responses. In this study, we evaluated the impact of CpG stimulation on precursor B-ALL cell lines and pediatric patient-derived samples. The ability to respond to CpG oligodeoxynucleotides was determined by the level of TLR9 expression. In contrast to both non-leukemic B cell precursors and mature B cells, the response of precursor B-ALL cells was characterized by increased CD40 expression but only small changes in CD86 levels and no induction of CD80 expression. CpG stimulation of ALL blasts produced increased levels of IL-6, IL-8 and IL-10 but no detectable IL-12p70 and led to a skewing of allogeneic T cells, with enhanced IFN- production and reduced secretion of IL-5. These results demonstrate the functional relevance CpG stimulation of precursor B lineage ALL cells and provide a rational basis for study of these agents for use in treatment of this disease. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: B. Varghese, A. Widman, J. Do, B. Taidi, D. K. Czerwinski, J. Timmerman, S. Levy, and R. Levy Generation of CD8+ T cell-mediated immunity against idiotype-negative lymphoma escapees Blood, November 12, 2009; 114(20): 4477 - 4485. [Abstract] [Full Text] [PDF] A. E. Seif, D. M. Barrett, M. Milone, V. I. Brown, S. A. Grupp, and G. S. D. Reid Long-term protection from syngeneic acute lymphoblastic leukemia by CpG ODN-mediated stimulation of innate and adaptive immune responses Blood, September 17, 2009; 114(12): 2459 - 2466. [Abstract] [Full Text] [PDF] D. Chiron, I. Bekeredjian-Ding, C. Pellat-Deceunynck, R. Bataille, and G. Jego Toll-like receptors: lessons to learn from normal and malignant human B cells Blood, September 15, 2008; 112(6): 2205 - 2213. [Abstract] [Full Text] [PDF] H. Fujii, J. D. Trudeau, D. T. Teachey, J. D. Fish, S. A. Grupp, K. R. Schultz, and G. S. D. Reid In vivo control of acute lymphoblastic leukemia by immunostimulatory CpG oligonucleotides Blood, March 1, 2007; 109(5): 2008 - 2013. [Abstract] [Full Text] [PDF]

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020