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Blood, 1 March 2005, Vol. 105, No. 5, pp. 1992-1999. Prepublished online as a Blood First Edition Paper on November 2, 2004; DOI 10.1182/blood-2004-07-2598. This Article Full Text (PDF) All Versions of this Article: 2004-07-2598v1 105/5/1992    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Murga, M. Articles by Tosato, G. Search for Related Content PubMed PubMed Citation Articles by Murga, M. Articles by Tosato, G. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 8, 2004 Accepted October 28, 2004 Neuropilin-1 regulates attachment in human endothelial cells independently of vascular endothelial growth factor receptor-2 Matilde Murga*, Oscar Fernandez-Capetillo, and Giovanna Tosato Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA * Corresponding author; email: costama{at}mail.nih.gov. Neuropilin-1 (NRP-1) is a type 1 membrane protein that binds the axon guidance factors belonging to the class-3 semaforin family. In endothelial cells, NRP-1 serves as a co-receptor for vascular endothelial growth factor (VEGF) and regulates VEGF receptor (R)-2-dependent angiogenesis. Though gene-targeting studies documenting embryonic lethality in NRP-1 null mice have demonstrated a critical role for NRP-1 in vascular development, the activities of NRP-1 in mature endothelial cells have been incompletely defined. Using RNA interference-mediated silencing of NRP-1 or VEGFR-2 in primary human endothelial cells we confirm that NRP-1 modulates VEGFR-2 signaling-dependent mitogenic functions of VEGF. Importantly, we now show that NRP-1 regulates endothelial cell adhesion to extra-cellular matrix proteins independently of VEGFR-2. Based on its dual role as an enhancer of VEGF activity and a mediator of endothelial cell adhesiveness described here, NRP-1 emerges as a promising molecular target for the development of anti-angiogenic drugs. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Sakakibara, C. A. Pise-Masison, J. N. Brady, and G. Tosato Gene Regulation and Functional Alterations Induced by Kaposi's Sarcoma-Associated Herpesvirus-Encoded ORFK13/vFLIP in Endothelial Cells J. Virol., March 1, 2009; 83(5): 2140 - 2153. [Abstract] [Full Text] [PDF] E. Sulpice, J. Plouet, M. Berge, D. Allanic, G. Tobelem, and T. Merkulova-Rainon Neuropilin-1 and neuropilin-2 act as coreceptors, potentiating proangiogenic activity Blood, February 15, 2008; 111(4): 2036 - 2045. [Abstract] [Full Text] [PDF] M. Narazaki, M. Segarra, and G. Tosato Neuropilin-2: A New Molecular Target for Antiangiogenic and Antitumor Strategies J Natl Cancer Inst, January 16, 2008; 100(2): 81 - 83. [Full Text] [PDF] T.-M. Hong, Y.-L. Chen, Y.-Y. Wu, A. Yuan, Y.-C. Chao, Y.-C. Chung, M.-H. Wu, S.-C. Yang, S.-H. Pan, J.-Y. Shih, et al. Targeting Neuropilin 1 as an Antitumor Strategy in Lung Cancer Clin. Cancer Res., August 15, 2007; 13(16): 4759 - 4768. [Abstract] [Full Text] [PDF] O. Salvucci, M. de la Luz Sierra, J. A. Martina, P. J. McCormick, and G. Tosato EphB2 and EphB4 receptors forward signaling promotes SDF-1-induced endothelial cell chemotaxis and branching remodeling Blood, November 1, 2006; 108(9): 2914 - 2922. [Abstract] [Full Text] [PDF] L. Wang, D. Mukhopadhyay, and X. Xu C terminus of RGS-GAIP-interacting protein conveys neuropilin-1-mediated signaling during angiogenesis FASEB J, July 1, 2006; 20(9): 1513 - 1515. [Abstract] [Full Text] [PDF] H. Jia, A. Bagherzadeh, B. Hartzoulakis, A. Jarvis, M. Lohr, S. Shaikh, R. Aqil, L. Cheng, M. Tickner, D. Esposito, et al. Characterization of a Bicyclic Peptide Neuropilin-1 (NP-1) Antagonist (EG3287) Reveals Importance of Vascular Endothelial Growth Factor Exon 8 for NP-1 Binding and Role of NP-1 in KDR Signaling J. Biol. Chem., May 12, 2006; 281(19): 13493 - 13502. [Abstract] [Full Text] [PDF] L. M. Ellis The role of neuropilins in cancer Mol. Cancer Ther., May 1, 2006; 5(5): 1099 - 1107. [Abstract] [Full Text] [PDF] D. H. Damon Vascular endothelial-derived semaphorin 3 inhibits sympathetic axon growth Am J Physiol Heart Circ Physiol, March 1, 2006; 290(3): H1220 - H1225. [Abstract] [Full Text] [PDF] C. K. Williams, J.-L. Li, M. Murga, A. L. Harris, and G. Tosato Up-regulation of the Notch ligand Delta-like 4 inhibits VEGF-induced endothelial cell function Blood, February 1, 2006; 107(3): 931 - 939. [Abstract] [Full Text] [PDF] M. J. Gray, J. S. Wey, A. Belcheva, M. F. McCarty, J. G. Trevino, D. B. Evans, L. M. Ellis, and G. E. Gallick Neuropilin-1 Suppresses Tumorigenic Properties in a Human Pancreatic Adenocarcinoma Cell Line Lacking Neuropilin-1 Coreceptors Cancer Res., May 1, 2005; 65(9): 3664 - 3670. [Abstract] [Full Text] [PDF]

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