SEARCH:   Advanced

Blood, 1 May 2005, Vol. 105, No. 9, pp. 3545-3551. Prepublished online as a Blood First Edition Paper on December 9, 2004; DOI 10.1182/blood-2004-07-2617. This Article Full Text (PDF) All Versions of this Article: 2004-07-2617v1 105/9/3545    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yu, G. Articles by Sachais, B. S Search for Related Content PubMed PubMed Citation Articles by Yu, G. Articles by Sachais, B. S Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 12, 2004 Accepted December 7, 2004 Endothelial expression of E-selectin is induced by the platelet specific chemokine Platelet Factor 4 through LRP in an NF-B dependent manner Guangyao Yu, Ann H Rux, Peihong Ma, Khalil Bdeir, and Bruce S Sachais* Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA * Corresponding author; email: sachais{at}mail.med.upenn.edu. The involvement of platelets in the pathogenesis of atherosclerosis has recently gained much attention. Platelet factor 4 (PF4), a platelet specific chemokine released upon platelet activation, has been localized to atherosclerotic lesions, including macrophages and endothelium. In this report, we demonstrate that E-selectin, an adhesion molecule involved in atherogenesis, is up-regulated in human umbilical vein endothelial cells exposed to PF4. Induction of E-selectin RNA is time and dose dependent. Surface expression of E-selectin, as measured by flow cytometry, is also increased by PF4. PF4 induces E-selectin expression by activation of transcriptional activity. Activation of NF-B is critical for PF4 induced E-selectin expression, as demonstrated by promoter activation studies and electrophoretic mobility shift assay. Further, we have identified the low density lipoprotein receptor-related protein (LRP) as the cell surface receptor mediating this effect. These results demonstrate that PF4 is able to increase expression of E-selectin by endothelial cells, and represents another potential mechanism by which platelets may participate in atherosclerotic lesion progression. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Y. Suzuki, N. Nagai, K. Yamakawa, J. Kawakami, H. R. Lijnen, and K. Umemura Tissue-type plasminogen activator (t-PA) induces stromelysin-1 (MMP-3) in endothelial cells through activation of lipoprotein receptor-related protein Blood, October 8, 2009; 114(15): 3352 - 3358. [Abstract] [Full Text] [PDF] M. P. Lambert, Y. Wang, K. H. Bdeir, Y. Nguyen, M. A. Kowalska, and M. Poncz Platelet factor 4 regulates megakaryopoiesis through low-density lipoprotein receptor-related protein 1 (LRP1) on megakaryocytes Blood, September 10, 2009; 114(11): 2290 - 2298. [Abstract] [Full Text] [PDF] H. Wang, W. Zhang, R. Tang, R. P. Hebbel, M. A. Kowalska, C. Zhang, J. D. Marth, M. Fukuda, C. Zhu, and Y. Huo Core2 1-6-N-Glucosaminyltransferase-I Deficiency Protects Injured Arteries From Neointima Formation in ApoE-Deficient Mice Arterioscler Thromb Vasc Biol, July 1, 2009; 29(7): 1053 - 1059. [Abstract] [Full Text] [PDF] J. I. Borissoff, H. M.H. Spronk, S. Heeneman, and H. ten Cate Is thrombin a key player in the 'coagulation-atherogenesis' maze? Cardiovasc Res, June 1, 2009; 82(3): 392 - 403. [Abstract] [Full Text] [PDF] J. M. van Gils, J. J. Zwaginga, and P. L. Hordijk Molecular and functional interactions among monocytes, platelets, and endothelial cells and their relevance for cardiovascular diseases J. Leukoc. Biol., February 1, 2009; 85(2): 195 - 204. [Abstract] [Full Text] [PDF] Y. Cai, J. Wang, R. Li, G. Ayala, M. Ittmann, and M. Liu GGAP2/PIKE-A Directly Activates Both the Akt and Nuclear Factor-{kappa}B Pathways and Promotes Prostate Cancer Progression Cancer Res., February 1, 2009; 69(3): 819 - 827. [Abstract] [Full Text] [PDF] R. M. Douglas and G. G. Haddad Can O2 Dysregulation Induce Premature Aging? Physiology, December 1, 2008; 23(6): 333 - 349. [Abstract] [Full Text] [PDF] K. Zurhove, C. Nakajima, J. Herz, H. H. Bock, and P. May {gamma}-Secretase Limits the Inflammatory Response Through the Processing of LRP1 Sci. Signal., November 25, 2008; 1(47): ra15 - ra15. [Abstract] [Full Text] [PDF] C. A. Gleissner, P. von Hundelshausen, and K. Ley Platelet Chemokines in Vascular Disease Arterioscler Thromb Vasc Biol, November 1, 2008; 28(11): 1920 - 1927. [Abstract] [Full Text] [PDF] G. Tabatabai, C. Herrmann, G. von Kurthy, M. Mittelbronn, S. Grau, B. Frank, R. Mohle, M. Weller, and W. Wick VEGF-dependent induction of CD62E on endothelial cells mediates glioma tropism of adult haematopoietic progenitor cells Brain, October 1, 2008; 131(10): 2579 - 2595. [Abstract] [Full Text] [PDF] M. Gritters, P. Borgdorff, M. P. C. Grooteman, M. Schoorl, M. Schoorl, P. C. M. Bartels, G.-J. Tangelder, and M. J. Nube Platelet activation in clinical haemodialysis: LMWH as a major contributor to bio-incompatibility? Nephrol. Dial. Transplant., September 1, 2008; 23(9): 2911 - 2917. [Abstract] [Full Text] [PDF] A. Gaultier, S. Arandjelovic, S. Niessen, C. D. Overton, M. F. Linton, S. Fazio, W. M. Campana, B. F. Cravatt III, and S. L. Gonias Regulation of tumor necrosis factor receptor-1 and the IKK-NF-{kappa}B pathway by LDL receptor-related protein explains the antiinflammatory activity of this receptor Blood, June 1, 2008; 111(11): 5316 - 5325. [Abstract] [Full Text] [PDF] G. Davi and C. Patrono Platelet Activation and Atherothrombosis N. Engl. J. Med., December 13, 2007; 357(24): 2482 - 2494. [Full Text] [PDF] E. Galkina and K. Ley Vascular Adhesion Molecules in Atherosclerosis Arterioscler Thromb Vasc Biol, November 1, 2007; 27(11): 2292 - 2301. [Abstract] [Full Text] [PDF] X. Zhang, R. Polavarapu, H. She, Z. Mao, and M. Yepes Tissue-Type Plasminogen Activator and the Low-Density Lipoprotein Receptor-Related Protein Mediate Cerebral Ischemia-Induced Nuclear Factor-{kappa}B Pathway Activation Am. J. Pathol., October 1, 2007; 171(4): 1281 - 1290. [Abstract] [Full Text] [PDF] M. P. Lambert, L. Rauova, M. Bailey, M. C. Sola-Visner, M. A. Kowalska, and M. Poncz Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications Blood, August 15, 2007; 110(4): 1153 - 1160. [Abstract] [Full Text] [PDF] C. Deiner, E. Shagdarsuren, P. L. Schwimmbeck, P. Rosenthal, C. Loddenkemper, U. Rauch, M. Pauschinger, R. Dietz, H.-P. Schultheiss, R. Dechend, et al. Nf-{kappa}b and AP-1 activation is associated with late lumen loss after porcine coronary angioplasty and antiproliferative beta-irradiation Cardiovasc Res, July 1, 2007; 75(1): 195 - 204. [Abstract] [Full Text] [PDF] S. Abele, M. Weyand, M. Wollin, N. E. Hiemann, F. Harig, T. Fischlein, and S. M. Ensminger Clopidogrel reduces the development of transplant arteriosclerosis J. Thorac. Cardiovasc. Surg., May 1, 2006; 131(5): 1161 - 1166. [Abstract] [Full Text] [PDF]

	Copyright © 2004 by American Society of Hematology         Online ISSN: 1528-0020