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Blood, 1 April 2005, Vol. 105, No. 7, pp. 2812-2820. Prepublished online as a Blood First Edition Paper on December 2, 2004; DOI 10.1182/blood-2004-07-2630. This Article Full Text (PDF) All Versions of this Article: 2004-07-2630v1 105/7/2812    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tiacci, E. Articles by Falini, B. Search for Related Content PubMed PubMed Citation Articles by Tiacci, E. Articles by Falini, B. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 15, 2004 Accepted November 17, 2004 Tumor protein D52 (TPD52): A novel B-cell/plasma cell molecule with unique expression pattern and Ca2+-dependent association with Annexin VI Enrico Tiacci, Pier-Luigi Orvietani, Barbara Bigerna, Alessandra Pucciarini, Garry L Corthals, Valentina Pettirossi, Maria P Martelli, Arcangelo Liso, Roberta Benedetti, Roberta Pacini, Niccolo Bolli, Stefano Pileri, Karen Pulford, Marcello Gambacorta, Antonino Carbone, Carla Pasquarello, Alexander Scherl, Helen Robertson, Maria T Sciurpi, Giovanni Alunni-Bistocchi, Luciano Binaglia, Jennifer A Byrne, and Brunangelo Falini* Institutes of Hematology and Biochemistry, University of Perugia, Perugia, Italy Geneva Proteomics Research Center, Geneva, Switzerland Institute of Hematology, University of Foggia, Foggia, Italy Institute of Pathology, Policlinico S. Orsola, Bologna, Italy LRF Immunodiagnostic Unit, John Radcliffe Hospital, Oxford, United Kingdom Institute of Pathology, Niguarda Hospital, Milan, Italy Division of Pathology, Oncology Reference Center, National Tumor Institute, Aviano, Italy Oncology Research Unit and the University of Sydney Department of Pediatrics and Child's Health, The Children's Hospital at Westmead, Westmead, NSW, Australia * Corresponding author; email: faliniem{at}unipg.it. We generated a murine monoclonal antibody (B28p) detecting an antigenic determinat shared by the IRTA1 receptor (the immunogen used to raise B28p) and an unrelated 28 kDa protein that was subsequently subjected to extensive characterization. The expression of the 28 kDa protein in normal lympho-hemopoietic tissues was restricted to B-cells and plasma cells, and clearly differed from that expected for IRTA1 (selectively expressed by MALT marginal zone B-cells). 2D-PAGE/mass-spectrometry analysis identified the 28 kDa protein as human TPD52, whose expression had been previously described only in normal and neoplastic epithelia. Specific B28p reactivity with TPD52 was confirmed by immunostaining/immunoblotting of TPD52-transfected cells. TPD52 expression pattern in normal and neoplastic B-cells was unique. In fact, unlike other B-cell molecules (PAX5, CD19, CD79a, CD20, CD22), which are down-regulated during differentiation from B cells to plasma cells, TPD52 expression reached its maximum levels at the plasma cell stage. In the Thiel myeloma cell line, TPD52 bound to annexin VI in a Ca+2-dependent manner, suggesting that these molecules, similarly to what observed in pancreatic acinar cells, may act in concert to regulate secretory processes in plasma cells. Finally, the anti-TPD52 monoclonal antibody served as a valuable tool for the diagnosis of B-cell malignancies. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. S. Chew, X. Chen, H. Zhang, E. A. Berg, and H. Zhang Calcium/calmodulin-dependent phosphorylation of tumor protein D52 on serine residue 136 may be mediated by CAMK2{delta}6 Am J Physiol Gastrointest Liver Physiol, December 1, 2008; 295(6): G1159 - G1172. [Abstract] [Full Text] [PDF] G. Bode, A. Luken, C. Kerkhoff, J. Roth, S. Ludwig, and W. Nacken Interaction between S100A8/A9 and Annexin A6 Is Involved in the Calcium-induced Cell Surface Exposition of S100A8/A9 J. Biol. Chem., November 14, 2008; 283(46): 31776 - 31784. [Abstract] [Full Text] [PDF] M. Shehata, I. Bieche, R. Boutros, J. Weidenhofer, S. Fanayan, L. Spalding, N. Zeps, K. Byth, R. K. Bright, R. Lidereau, et al. Nonredundant Functions for Tumor Protein D52-Like Proteins Support Specific Targeting of TPD52 Clin. Cancer Res., August 15, 2008; 14(16): 5050 - 5060. [Abstract] [Full Text] [PDF] J. D. Lewis, L. A. Payton, J. G. Whitford, J. A. Byrne, D. I. Smith, L. Yang, and R. K. Bright Induction of Tumorigenesis and Metastasis by the Murine Orthologue of Tumor Protein D52 Mol. Cancer Res., February 1, 2007; 5(2): 133 - 144. [Abstract] [Full Text] [PDF]

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