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Blood, 1 August 2005, Vol. 106, No. 3, pp. 978-987. Prepublished online as a Blood First Edition Paper on April 19, 2005; DOI 10.1182/blood-2004-07-2656. This Article Full Text (PDF) All Versions of this Article: 2004-07-2656v1 106/3/978    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Orinska, Z. Articles by Bulfone-Paus, S. Search for Related Content PubMed PubMed Citation Articles by Orinska, Z. Articles by Bulfone-Paus, S. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 13, 2004 Accepted April 4, 2005 TLR3-induced activation of mast cells modulates CD8+ T cell-recruitment Zane Orinska, Elena Bulanova, Vadim Budagian, Martin Metz, Marcus Maurer, and Silvia Bulfone-Paus* Deprtment of Immunology and Cell Biology, Research Center Borstel, Borstel, Germany Department of Dermatology, University of Mainz, Mainz, Germany Department of Dermatology, University of Mainz, Mainz, Germany; Department of Dermatology and Allergy, University Hospital Charite, Humboldt University Berlin, Berlin, Germany * Corresponding author; email: sbulfone{at}fz-borstel.de. Mast cells are known to play an important role in host defense against various pathogens, but their role in viral infection has not been clarified in detail. Double-stranded RNA, synthesised by various types of viruses and mimicked by poly(I:C), is recognized by Toll-like receptor 3 (TLR3). In this study, we demonstrate that poly(I:C) injection in vivo potently stimulates peritoneal mast cells to upregulate a number of different co-stimulatory molecules. Therefore, we examined the expression and the functional significance of TLR3 activation in mast cells. Mast cells express TLR3 on the cell surface and intracellularly. After stimulation of mast cells with poly(I:C) and Newcastle disease virus (NDV), TLR3 is phosphorylated and the expression of key antiviral response cytokines (IFN, ISG15) and chemokines (IP10, RANTES) is upregulated. Interestingly, mast cells activated via TLR3-poly(I:C) potently stimulate CD8+ T cell recruitment. Indeed, mast cell deficient mice (KitW/KitW-v) intraperitoneally injected with poly(I:C) show a decreased CD8+ T cell recruitment, whereas granulocytes normally migrate to the peritoneal cavity. Mast cell-reconstitution of KitW/KitW-v mice normalizes the CD8+ T cell influx. Thus, mast cells stimulated through engagement of TLR3 are potent regulators of CD8+ T cell activities in vitro and in vivo. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. Bulanova, V. Budagian, Z. Orinska, F. Koch-Nolte, F. Haag, and S. Bulfone-Paus ATP induces P2X7 receptor-independent cytokine and chemokine expression through P2X1 and P2X3 receptors in murine mast cells J. Leukoc. Biol., April 1, 2009; 85(4): 692 - 702. [Abstract] [Full Text] [PDF] Q. Sun, W. Li, R. She, D. Wang, D. Han, R. Li, Y. Ding, and Z. Yue Evidence for a role of mast cells in the mucosal injury induced by Newcastle disease virus Poult. Sci., March 1, 2009; 88(3): 554 - 561. [Abstract] [Full Text] [PDF] S. M. Burke, T. B. Issekutz, K. Mohan, P. W. K. Lee, M. Shmulevitz, and J. S. Marshall Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism Blood, June 15, 2008; 111(12): 5467 - 5476. [Abstract] [Full Text] [PDF] J. N. Leonard, R. Ghirlando, J. Askins, J. K. Bell, D. H. Margulies, D. R. Davies, and D. M. Segal The TLR3 signaling complex forms by cooperative receptor dimerization PNAS, January 8, 2008; 105(1): 258 - 263. [Abstract] [Full Text] [PDF] E. Vercammen, J. Staal, and R. Beyaert Sensing of Viral Infection and Activation of Innate Immunity by Toll-Like Receptor 3 Clin. Microbiol. Rev., January 1, 2008; 21(1): 13 - 25. [Abstract] [Full Text] [PDF] V. Heib, M. Becker, T. Warger, G. Rechtsteiner, C. Tertilt, M. Klein, T. Bopp, C. Taube, H. Schild, E. Schmitt, et al. Mast cells are crucial for early inflammation, migration of Langerhans cells, and CTL responses following topical application of TLR7 ligand in mice Blood, August 1, 2007; 110(3): 946 - 953. [Abstract] [Full Text] [PDF] M. G. Brown, C. A. King, C. Sherren, J. S. Marshall, and R. Anderson A dominant role for Fc{gamma}RII in antibody-enhanced dengue virus infection of human mast cells and associated CCL5 release J. Leukoc. Biol., December 1, 2006; 80(6): 1242 - 1250. [Abstract] [Full Text] [PDF] G. E. Morris, L. C. Parker, J. R. Ward, E. C. Jones, M. K. B. Whyte, C. E. Brightling, P. Bradding, S. K. Dower, and I. Sabroe Cooperative molecular and cellular networks regulate Toll-like receptor-dependent inflammatory responses FASEB J, October 1, 2006; 20(12): 2153 - 2155. [Abstract] [Full Text] [PDF]

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