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Blood, 15 February 2005, Vol. 105, No. 4, pp. 1823-1827. Prepublished online as a Blood First Edition Paper on October 21, 2004; DOI 10.1182/blood-2004-07-2832. This Article Full Text (PDF) All Versions of this Article: 2004-07-2832v1 105/4/1823    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mommaas, B. Articles by Goulmy, E. Search for Related Content PubMed PubMed Citation Articles by Mommaas, B. Articles by Goulmy, E. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 26, 2004 Accepted October 7, 2004 Cord blood comprises antigen-experienced T cells specific for maternal minor histocompatibility antigen HA-1 Bregje Mommaas, Janine A Stegehuis-Kamp, Astrid G van Halteren, Michel Kester, Jurgen Enczmann, Peter Wernet, Gesine Kogler, Tuna Mutis, Anneke Brand, and Els Goulmy* Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands Heinrich Heine University, Institute for Transplantation Diagnostics and Cell Therapeutics, Dusseldorf, Germany Sanquin Blood Bank, Leiden, The Netherlands * Corresponding author; email: E.A.J.M.Goulmy{at}lumc.nl. Umbilical cord blood transplantation is applied as treatment for mainly pediatric patients with hematological malignancies. The clinical results show a relatively low incidence of Graft-versus-Host-Disease and leukemia relapse. Since maternal cells traffic into the fetus during pregnancy, we questioned whether cord blood has the potential to generate cytotoxic T cells specific for the hematopoietic minor Histocompatibility (H) antigen HA-1 that would support the Graft-versus-Leukemia effect. Here we demonstrate the feasibility of ex-vivo generation of minor H antigen HA-1 specific T cells from cord blood cells. Moreover, we observed already pre-existing HA-1 specific T cells in cord blood samples. Both the circulating and the ex-vivo generated HA-1 specific T cells show specific and hematopoietic restricted lysis of HLA-A2pos/HA-1pos target cells, including leukemic cells. The cord blood derived HA-1 specific cytotoxic T cells are from child origin. Thus, the so-called naive cord blood can comprise cytotoxic T cells directed at the maternal minor H antigen HA-1. The apparent immunization status of cord blood may well contribute to the in vivo Graft-versus-Leukemia activity after transplantation. Moreover, since the fetus cannot be primed against Y chromosome encoded minor H antigens, cord blood is an attractive stem cell source for male patients. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. J. van Rood, C. E. Stevens, J. Smits, C. Carrier, C. Carpenter, and A. Scaradavou Reexposure of cord blood to noninherited maternal HLA antigens improves transplant outcome in hematological malignancies PNAS, November 24, 2009; 106(47): 19952 - 19957. [Abstract] [Full Text] [PDF] P. J. Hanley, C. R. Y. Cruz, B. Savoldo, A. M. Leen, M. Stanojevic, M. Khalil, W. Decker, J. J. Molldrem, H. Liu, A. P. Gee, et al. 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Huizinga, et al. Protective effect of noninherited maternal HLA-DR antigens on rheumatoid arthritis development PNAS, December 11, 2007; 104(50): 19966 - 19970. [Abstract] [Full Text] [PDF] A. Mullally and J. Ritz Beyond HLA: the significance of genomic variation for allogeneic hematopoietic stem cell transplantation Blood, February 15, 2007; 109(4): 1355 - 1362. [Abstract] [Full Text] [PDF] K. Schilbach, G. Kerst, S. Walter, M. Eyrich, D. Wernet, R. Handgretinger, W. Xie, H.-G. Rammensee, I. Muller, H.-J. Buhring, et al. Cytotoxic minor histocompatibility antigen HA-1-specific CD8+ effector memory T cells: artificial APCs pave the way for clinical application by potent primary in vitro induction Blood, July 1, 2005; 106(1): 144 - 149. [Abstract] [Full Text] [PDF] W. Tse and M. J. Laughlin Umbilical Cord Blood Transplantation: A New Alternative Option Hematology, January 1, 2005; 2005(1): 377 - 383. [Abstract] [Full Text] [PDF]

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