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Blood, 1 August 2005, Vol. 106, No. 3, pp. 893-898. Prepublished online as a Blood First Edition Paper on April 21, 2005; DOI 10.1182/blood-2004-07-2859. This Article Full Text (PDF) Supplemental Table All Versions of this Article: 2004-07-2859v1 106/3/893    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Glimm, H. Articles by von Kalle, C. Search for Related Content PubMed PubMed Citation Articles by Glimm, H. Articles by von Kalle, C. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 26, 2004 Accepted April 11, 2005 Efficient marking of human cells with rapid but transient repopulating activity in autografted recipients Hanno Glimm, Manfred Schmidt, Marlene Fischer, Kerstin Schwarzwaelder, Manuela Wissler, Silke Klingenberg, Claudia Prinz, Cornelius F Waller, Winand Lange, Connie J Eaves*, and Christof von Kalle Department of Internal Medicine I, Albert-Ludwigs-University, Freiburg, Germany; Institute of Molecular Medicine and Cell Research, Albert-Ludwigs-University, Freiburg, Germany Department of Internal Medicine I, Albert-Ludwigs-University, Freiburg, Germany Johanniter Hospital Rheinhausen, Duisburg, Germany Terry Fox Laboratory, British Columbia Cancer Agency and Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada Experimental Hematology Research Foundation, Molecular and Gene Therapy Program, Cincinnati Children's Hospital, Cincinnati, OH, USA * Corresponding author; email: ceaves{at}bccrc.ca. Short term hematopoietic reconstituting cells have been identified in mice, non-human primates and amongst human cells that engraft xenogeneic hosts. We now present clonal marking data demonstrating a rapid but unsustained contribution of cultured human autografts to the initial phase of hematologic recovery in myeloablated patients. Three patients were transplanted with granulocyte colony-stimulating factor-mobilized autologous peripheral blood (PB) cells, of which a portion (8-25% of the CD34+ cells) had been incubated in vitro with growth factors (5 days) and clinical grade LN-retrovirus (3-5 days). More than 9% of the clonogenic and longterm culture-initiating cells harvested were transduced. Semi-quantitative and linear amplification-mediated PCR analyses of serial PB samples showed that marked WBCs appeared in all 3 patients within 11 days and transiently constituted up to 0.1-1% of those produced in the first month. However, within another 2-9 months, marked cells had permanently decreased to very low levels. Analysis of >50 vector insertion sites showed none of the clones detected in the first month were active later. 80% of inserts were located within or near genes, 2 near CXCR4. These findings provide direct evidence of cells with rapid but transient repopulating activity in patients and demonstrate their efficient transduction in vitro. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: P. A. Horn, H.-P. Kiem, K. Ando, and T. Yahata Expansion of SCID repopulating cells does not prove expansion of hematopoietic stem cells. Blood, July 15, 2006; 108(2): 771 - 772. [Full Text] [PDF]

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