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Blood, 1 July 2005, Vol. 106, No. 1, pp. 144-149. Prepublished online as a Blood First Edition Paper on February 24, 2005; DOI 10.1182/blood-2004-07-2940. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: 2004-07-2940v1 106/1/144    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Schilbach, K. Articles by Niethammer, D. Search for Related Content PubMed PubMed Citation Articles by Schilbach, K. Articles by Niethammer, D. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 29, 2004 Accepted February 11, 2005 Cytotoxic minor histocompatibility antigen HA-1-specific CD8+ effector memory T cells: Artificial APCs pave the way for clinical application by potent primary in vitro induction Karin Schilbach*, Gunter Kerst, Steffen Walter, Matthias Eyrich, Dorothee Wernet, Rupert Handgretinger, Weidong Xie, Hans-Georg Rammensee, Ingo Muller, Hans-Jorg Buhring, and Dietrich Niethammer University Children's Hospital Tubingen, Tubingen, Germany Department of Immunology, Institute for Cell Biology, University of Tubingen, Tubingen, Germany Department of Transfusion Medicine, University Hospital Tubingen, Tubingen, Germany St. Jude Children's Research Hospital, Memphis, TN, USA Department of Hematology and Oncology, University Hospital Tubingen, Tubingen, Germany * Corresponding author; email: knschilb{at}med.uni-tuebingen.de. Induction of cytotoxic T lymphocytes (CTLs) for treatment of relapsed leukemia after allogeneic stem cell transplantation is hindered by the laborious and time-consuming procedure of generating dendritic cells for antigen presentation. Artificial antigen-presenting cells (aAPCs) offer the advantage of being readily available in sufficient numbers, thus allowing for a highly standardized in vitro induction of CTLs. We generated aAPCs coated with anti-CD28-Ab and either high-density (HD) or low-density (LD) MHC class I molecules loaded with a nonapeptide derived from the hematopoiesis-restricted minor histocompatibility antigen HA-1H. HD- and LD-aAPCs potently induced HA-1H-specific CD8+ CTLs from untouched CD8+ T cells of healthy donors. CTLs were subsequently purified by magnetic activated cell sorting. HD- as well as LD-aAPC-induced CTLs exerted high HA-1H-specific cytotoxicity, resembled Tc1 effector memory cells, survived long time in vitro and were expanded by a factor varying between 8.2 and 51x104. T-cell receptor (TCR) repertoire of HA-1-tetramer+ CTLs was oligoclonal with a prominent usage of V6. The TCR repertoire of tetramer+ CTLs was distinct from and more restricted than that of tetramer- cells. These findings indicate that aAPCs are attractive tools for the ex vivo generation of HA-1-specific CTLs suitable for immunotherapy of relapsed leukemia. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. Zappasodi, M. Di Nicola, C. Carlo-Stella, R. Mortarini, A. Molla, C. Vegetti, S. Albani, A. Anichini, and A. M. Gianni The effect of artificial antigen-presenting cells with preclustered anti-CD28/-CD3/-LFA-1 monoclonal antibodies on the induction of ex vivo expansion of functional human antitumor T cells Haematologica, October 1, 2008; 93(10): 1523 - 1534. [Abstract] [Full Text] [PDF] M. Ditschkowski, A. H. Elmaagacli, R. Trenschel, R. Peceny, M. Koldehoff, C. Schulte, and D. W. Beelen T-cell depletion prevents from bronchiolitis obliterans and bronchiolitis obliterans with organizing pneumonia after allogeneic hematopoietic stem cell transplantation with related donors Haematologica, April 1, 2007; 92(4): 558 - 561. [Abstract] [Full Text] [PDF]

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