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Blood, 15 April 2005, Vol. 105, No. 8, pp. 3330-3339. Prepublished online as a Blood First Edition Paper on December 23, 2004; DOI 10.1182/blood-2004-08-2988. This Article Full Text (PDF) All Versions of this Article: 2004-08-2988v1 105/8/3330    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wen, J. Articles by Noguchi, C. T Search for Related Content PubMed PubMed Citation Articles by Wen, J. Articles by Noguchi, C. T Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted August 9, 2004 Accepted December 10, 2004 SATB1 family protein expressed during early erythroid differentiation modifies globin gene expression Jie Wen, Suming Huang, Heather Rogers, Liliane A Dickinson, Terumi Kohwi-Shigematsu, and Constance T Noguchi* Laboratory of Chemical Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA Department of Cell and Molecular Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, USA * Corresponding author; email: cnoguchi{at}helix.nih.gov. SATB1 nuclear protein, expressed predominantly in T-cells, regulates genes through targeting chromatin remodeling during T-cell maturation. Here we show SATB1 family protein induction during early human adult erythroid progenitor cell differentiation concomitant with -globin expression. Erythroid differentiation of human erythroleukemia K562 cells by hemin simultaneously increases -globin and downregulates SATB1 family protein and -globin gene expression. Chromatin immunoprecipitation (ChIP) using anti-SATB1 antibody shows selective binding in vivo in the -globin cluster to the hypersensitive site 2 (HS2) in the locus control region (LCR) and to the -globin promoter. SATB1 overexpression increases -globin and decreases-globin gene expression accompanied by histone hyperacetylation and hypomethylation in chromatin from the -globin promoter and HS2, and histone hypoacetylation and hypermethylation associated with the -globin promoter. In K562 cells SATB1 family protein forms a complex with CREB-binding protein (CBP) important in transcriptional activation. In co-transfection experiments, increase in -promoter activity by SATB1 was amplified by CBP and blocked by E1A, a CBP inhibitor. Our results suggest that SATB1 can upregulate the -globin gene by interaction with specific sites in the -globin cluster and imply that SATB1 family protein expressed in the erythroid progenitor cells may have a role in globin gene expression during early erythroid differentiation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Xu and P. R. Cook Similar active genes cluster in specialized transcription factories J. Cell Biol., May 19, 2008; 181(4): 615 - 623. [Abstract] [Full Text] [PDF] P. K. Purbey, S. Singh, P. P. Kumar, S. Mehta, K. N. Ganesh, D. Mitra, and S. Galande PDZ domain-mediated dimerization and homeodomain-directed specificity are required for high-affinity DNA binding by SATB1 Nucleic Acids Res., April 1, 2008; 36(7): 2107 - 2122. [Abstract] [Full Text] [PDF] K. Yamasaki, T. Akiba, T. Yamasaki, and K. Harata Structural basis for recognition of the matrix attachment region of DNA by transcription factor SATB1 Nucleic Acids Res., August 1, 2007; 35(15): 5073 - 5084. [Abstract] [Full Text] [PDF] R. P. Martins and S. A. Krawetz Decondensing the protamine domain for transcription PNAS, May 15, 2007; 104(20): 8340 - 8345. [Abstract] [Full Text] [PDF] R. Ajay Kumar, S. R. Naidu, X. Wang, A. N. Imbalzano, and E. J. Androphy Interaction of Papillomavirus E2 Protein with the Brm Chromatin Remodeling Complex Leads to Enhanced Transcriptional Activation J. Virol., March 1, 2007; 81(5): 2213 - 2220. [Abstract] [Full Text] [PDF] J. A. McNeil, K. P. Smith, L. L. Hall, and J. B. Lawrence Word frequency analysis reveals enrichment of dinucleotide repeats on the human X chromosome and [GATA]n in the X escape region Genome Res., April 1, 2006; 16(4): 477 - 484. [Abstract] [Full Text] [PDF] H. Yamaguchi, M. Tateno, and K. Yamasaki Solution Structure and DNA-binding Mode of the Matrix Attachment Region-binding Domain of the Transcription Factor SATB1 That Regulates the T-cell Maturation J. Biol. Chem., February 24, 2006; 281(8): 5319 - 5327. [Abstract] [Full Text] [PDF]

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