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Blood, 1 April 2005, Vol. 105, No. 7, pp. 2787-2792.
Prepublished online as a Blood First Edition Paper on December 14, 2004; DOI 10.1182/blood-2004-09-3388.
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Submitted September 1, 2004
Accepted October 17, 2004
Ly49Q defines two pDC subsets in mice
Yumiko Kamogawa-Schifter*, Jun Ohkawa, Sahori Namiki, Naoko Arai, Ken-ichi Arai, and YongJun Liu
Department of Immunobiology, Ginkgo Biomedical Research Institute, Tokyo, Japan; Department of Molecular and Developmental Biology, Insitute of Medical Science University of Tokyo, Tokyo, Japan
Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Department of Immunology, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA
* Corresponding author; email: kamogawa{at}ginkgobio.co.jp.
Plasmacytoid dendritic cells (pDCs) play an important primary role for anti-viral innate immunity by rapidly producing large amounts of type 1 interferon (IFN) upon viral infection. To study pDC biology, we generated a monoclonal antibody, termed 2E6, that recognizes pDCs. Molecular cloning of a cDNA encoding the 2E6 antigen revealed that it is a type II C-type lectin, Ly49Q, that consists of 247 amino acids with high homology to the NK receptor family Ly49, with an immunoreceptor tyrosine-based inhibitory motif in the cytoplasmic domain. Ly49Q is expressed on pDCs but not on NK cells, and on myeloid dendritic cells. B220+, CD11c+, CD11b- pDCs in bone marrow were divided into Ly49Q+ and Ly49Q- subsets. While both subsets produced IFN- upon CpG and herpes simplex virus stimulation, Ly49Q- pDCs responded poorly to influenza virus. In addition, Ly49Q- pDCs produced inflammatory cytokines such as IL-6, IL-12, and TNF- upon stimulation at lower levels than those produced by Ly49Q+ pDCs. In contrast to bone marrow, Ly49Q+pDCs were only found in peripheral blood, lymph node, and spleen. These results indicate that Ly49Q is a specific marker for peripheral pDCs and that expression of Ly49Q defines two subsets of pDCs in bone marrow.
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