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Blood, 1 March 2005, Vol. 105, No. 5, pp. 1937-1945. Prepublished online as a Blood First Edition Paper on November 2, 2004; DOI 10.1182/blood-2004-09-3459. This Article Full Text (PDF) All Versions of this Article: 2004-09-3459v1 105/5/1937    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Terszowski, G. Articles by Rodewald, H.-R. Search for Related Content PubMed PubMed Citation Articles by Terszowski, G. Articles by Rodewald, H.-R. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 8, 2004 Accepted October 27, 2004 Prospective isolation and global gene expression analysis of the colony-forming unit-erythrocyte (CFU-E) Grzegorz Terszowski, Claudia Waskow, Peter Conradt, Dido Lenze, Jessica Koenigsmann, Dirk Carstanjen, Ivan Horak, and Hans-Reimer Rodewald* Dept. for Immunology, University of Ulm, Ulm, Germany Institute of Pathology, Charite, Benjamin Franklin University Hospital, Berlin, Germany Institute of Molecular Pharmacology, Dept. of Molecular Genetics, Berlin, Germany * Corresponding author; email: hans-reimer.rodewald{at}medizin.uni-ulm.de. The colony-forming unit-erythrocyte (CFU-E) is a rare bone marrow (BM) progenitor which generates erythrocyte colonies in 48 hours. The existence of CFU-E is based on these colonies but CFU-E have not been purified prospectively by phenotype. We have separated the 'non-stem', 'non-lymphoid' compartment (lineage marker [lin]-c-Kit+Sca-1-IL-7R-) into interleukin-3 receptor (IL-3R)- and IL-3R+ subsets. Within IL-3R-, but not IL-3R+ cells we have identified TER119-CD41-CD71+ erythrocyte-committed progenitors (EP). EP generate CFU-E colonies at ~70% efficiency, and generate reticulocytes in vivo. Depletion of EP from BM strongly reduces CFU-E frequencies. EP lack potential for burst-forming unit-erythrocyte, megakaryocyte, granulocyte (G) and monocyte (M) colonies, and for colony-forming unit-spleen. Chronically suppressed erythropoiesis in interferon consensus sequence binding protein (ICSBP)-deficient BM is associated with reduced frequencies of both the EP population and CFU-E colonies. During phenylhydrazine-induced acute anemia, numbers of both the EP population and CFU-E colonies increase. Collectively, EP (lin-c-Kit+Sca-1-IL-7R-IL-3R-CD41-CD71+) account for most, if not all CFU-E activity in BM. As a first molecular characterization, we have compared global gene expression in EP and non-erythroid GM progenitors. These analyses define an erythroid progenitor-specific gene expression pattern. The prospective isolation of EP is an important step to analyze physiological and pathological erythropoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Koenigsmann, C. Rudolph, S. Sander, O. Kershaw, A. D. Gruber, L. Bullinger, B. Schlegelberger, and D. Carstanjen Nf1 haploinsufficiency and Icsbp deficiency synergize in the development of leukemias Blood, May 7, 2009; 113(19): 4690 - 4701. [Abstract] [Full Text] [PDF] G. Chan, D. Kalaitzidis, T. Usenko, J. L. Kutok, W. Yang, M. G. Mohi, and B. G. Neel Leukemogenic Ptpn11 causes fatal myeloproliferative disorder via cell-autonomous effects on multiple stages of hematopoiesis Blood, April 30, 2009; 113(18): 4414 - 4424. 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