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Blood, 15 July 2005, Vol. 106, No. 2, pp. 419-427. Prepublished online as a Blood First Edition Paper on March 22, 2005; DOI 10.1182/blood-2004-09-3507. This Article Full Text (PDF) All Versions of this Article: 2004-09-3507v1 106/2/419    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sordi, V. Articles by Piemonti, L. Search for Related Content PubMed PubMed Citation Articles by Sordi, V. Articles by Piemonti, L. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 9, 2004 Accepted March 16, 2005 Bone marrow mesenchymal stem cells express a restricted set of functionally active chemokine receptors capable of promoting migration to pancreatic islets Valeria Sordi, Maria L Malosio, Federica Marchesi, Alessia Mercalli, Raffaella Melzi, Tiziana Giordano, Nathalie Belmonte, Giuliana Ferrari, Biagio E Leone, Federico Bertuzzi, Gianpaolo Zerbini, Paola Allavena, Ezio Bonifacio, and Lorenzo Piemonti* Telethon-Juvenile Diabetes Research Foundation Center for Cell Replacement, San Raffaele Scientific Institute, Milan, Italy Department of Immunology and Cell Biology, Mario Negri Institute, Milan, Italy H.S. Raffaele-Telethon Institute for Gene Therapy (HSR-TIGET), San Raffaele Scientific Institute, Milan, Italy Department of Surgery, University of Milano-Bicocca, Milan, Italy Renal Pathophysiology Laboratory, Division of Medicine, San Raffaele Scientific Institute, Milan, Italy * Corresponding author; email: piemonti.lorenzo{at}hsr.it. Bone marrow-derived mesenchymal stem cells (BM-MSCs) are stromal cells with the ability to proliferate and differentiate into many tissues. Although they represent powerful tools for several therapeutic settings, mechanisms regulating their migration to peripheral tissues are still unknown. Here, we report chemokine receptor expression on human BM-MSCs and their role in mediating migration to tissues. A minority of BM-MSCs (2 to 25%) expressed a restricted set of chemokine receptors (CXCR4, CX3CR1, CXCR6, CCR1, CCR7) and, accordingly, showed appreciable chemotactic migration in response to the chemokines CXCL12, CX3CL1, CXCL16, CCL3, and CCL19. Using human pancreatic islets as an in vitro model of peripheral tissue, we showed that islet supernatants released factors able to attract BM-MSCs in vitro, and this attraction was principally mediated by CX3CL1 and CXCL12. Moreover, cells with features of BM-MSCs were detected within the pancreatic islets of mice injected with GFP positive BM. A population of bona fide MSCs that also expressed CXCR4, CXCR6, CCR1, and CCR7 could be isolated from normal adult human pancreas. This study defines the chemokine receptor repertoire of human BM-MSCs which determines their migratory activity. Modulation of homing capacity may be instrumental for harnessing the therapeutic potential of BM-MSCs. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Tommila, A. Jokilammi, P. Terho, T. Wilson, R. Penttinen, and E. 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