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Blood, 15 April 2005, Vol. 105, No. 8, pp. 3094-3099. Prepublished online as a Blood First Edition Paper on December 23, 2004; DOI 10.1182/blood-2004-09-3515. This Article Full Text (PDF) All Versions of this Article: 2004-09-3515v1 105/8/3094    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dhillon, N. K Articles by Buch, S. Search for Related Content PubMed PubMed Citation Articles by Dhillon, N. K Articles by Buch, S. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 10, 2004 Accepted December 20, 2004 Inhibition of pathogenic SHIV replication in macaques treated with antisense DNA of interleukin-4 Navneet K Dhillon, Yongjun Sui, Raghava Potula, Sukhbir Dhillon, Istvan Adany, Zhuang Li, Francois Villinger, David Pinson, Opendra Narayan, and Shilpa Buch* Department of Microbiology,Immunology and Molecular Genetics, Marion Merrell Dow Laboratory of Viral Pathogenesis, University of Kansas Medical Center, Kansas City, KS, USA Department of Pathology, Emory University, Atlanta, GA, USA * Corresponding author; email: sbuch{at}kumc.edu. Interleukin-4 is implicated in the pathogenesis of HIV-induced AIDS, and causes enhancement of replication of virus strains that utilize the CXCR4 (X4) co-receptor. In this study, we explored the effects of IL-4 antisense (AS) DNA on replication of X4, simian human immunodeficiency viruses, SHIVKU-2 and SHIV89.6P. AS IL-4 oligomer caused inhibition of virus replication in cultures of CD4+ T cells and macrophages derived from macaques. Plasmid expressing AS IL-4 DNA was also effective in abrogating virus replication in macrophage cultures. Relevance of these cell culture studies was confirmed in vivo by treating SHIV89.6P-infected macaques with AS IL-4 DNA. Six macaques were inoculated with the virus and four were treated with AS IL-4 DNA. This resulted in a significant decrease in viral RNA concentrations in the liver, lungs and spleen, tissues that are all sites of virus replication in macrophages. This is the first demonstration of effective inhibition of an HIV-like virus in tissues by AS DNA of a cytokine. In the present era of increasing resistance of HIV to antiviral compounds, exploration of adjunct therapies directed at host responses in combination with antiretroviral drugs may be of value for the treatment of AIDS. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. K. Dhillon, D. Pinson, S. Dhillon, O. Tawfik, M. Danley, M. Davis, O. Nemon, M. Mayo, A. Kumar, Y.-J. Tsai, et al. Bleomycin treatment causes enhancement of virus replication in the lungs of SHIV-infected macaques Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1233 - L1240. [Abstract] [Full Text] [PDF]

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