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Blood, 1 April 2005, Vol. 105, No. 7, pp. 2852-2861. Prepublished online as a Blood First Edition Paper on December 9, 2004; DOI 10.1182/blood-2004-09-3606. This Article Full Text (PDF) Supplemental Videos All Versions of this Article: 2004-09-3606v1 105/7/2852    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Barreiro, O. Articles by Sanchez-Madrid, F. Search for Related Content PubMed PubMed Citation Articles by Barreiro, O. Articles by Sanchez-Madrid, F. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 17, 2004 Accepted November 26, 2004 Endothelial tetraspanin microdomains regulate leukocyte firm adhesion during extravasation Olga Barreiro, Maria Yanez-Mo, Monica Sala-Valdes, Maria Dolores Gutierrez-Lopez, Susana Ovalle, Adrian Higginbottom, Peter N Monk, Carlos Cabanas, and Francisco Sanchez-Madrid* Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, Madrid, Spain Instituto de Farmacologia y Toxicologia CSIC-UCM, Facultad de Medicina, Universidad Complutense, Madrid, Spain Department of Academic Neurology, The Medical School, University of Sheffield, Sheffield, United Kingdom * Corresponding author; email: fsanchez.hlpr{at}salud.madrid.org. Tetraspanins associate with several transmembrane proteins forming microdomains involved in intercellular adhesion and migration. Here, we show that endothelial tetraspanins relocalize to the contact site with transmigrating leukocytes and associate laterally with both ICAM-1 and VCAM-1. Alteration of endothelial tetraspanin microdomains by CD9-LEL-GST peptides or CD9/CD151 siRNA oligonucleotides, interfered with ICAM-1 and VCAM-1 function preventing lymphocyte transendothelial migration and increasing lymphocyte detachment under shear flow. Heterotypic intercellular adhesion mediated by VCAM-1 or ICAM-1 was augmented when expressed exogenously in the appropriate tetraspanin environment. Therefore, tetraspanin microdomains have a crucial role in the proper adhesive function of ICAM-1 and VCAM-1 during leukocyte adhesion and transendothelial migration. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: O. Barreiro, R. J. Aguilar, E. Tejera, D. Megias, F. de Torres-Alba, A. Evangelista, and F. Sanchez-Madrid Specific Targeting of Human Inflamed Endothelium and In Situ Vascular Tissue Transfection by the Use of Ultrasound Contrast Agents J. Am. Coll. Cardiol. Img., August 1, 2009; 2(8): 997 - 1005. [Abstract] [Full Text] [PDF] F. Zhang, J. Kotha, L. K. Jennings, and X. A. Zhang Tetraspanins and vascular functions Cardiovasc Res, July 1, 2009; 83(1): 7 - 15. [Abstract] [Full Text] [PDF] J. Rohlena, O. L. Volger, J. D. van Buul, L. H.P. Hekking, J. M. van Gils, P. I. Bonta, R. D. Fontijn, J. A. Post, P. L. Hordijk, and A. J.G. Horrevoets Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion Cardiovasc Res, January 1, 2009; 81(1): 187 - 196. [Abstract] [Full Text] [PDF] G. Baldwin, V. Novitskaya, R. Sadej, E. Pochec, A. Litynska, C. Hartmann, J. Williams, L. Ashman, J. A. Eble, and F. Berditchevski Tetraspanin CD151 Regulates Glycosylation of {alpha}3{beta}1 Integrin J. Biol. Chem., December 19, 2008; 283(51): 35445 - 35454. [Abstract] [Full Text] [PDF] E. Kanters, J. van Rijssel, P. J. Hensbergen, D. Hondius, F. P. J. Mul, A. M. Deelder, A. Sonnenberg, J. D. van Buul, and P. L. Hordijk Filamin B Mediates ICAM-1-driven Leukocyte Transendothelial Migration J. Biol. Chem., November 14, 2008; 283(46): 31830 - 31839. [Abstract] [Full Text] [PDF] O. Barreiro, M. Zamai, M. Yanez-Mo, E. Tejera, P. Lopez-Romero, P. N. Monk, E. Gratton, V. R. Caiolfa, and F. Sanchez-Madrid Endothelial adhesion receptors are recruited to adherent leukocytes by inclusion in preformed tetraspanin nanoplatforms J. Cell Biol., November 3, 2008; 183(3): 527 - 542. [Abstract] [Full Text] [PDF] K. Ley and H. Zhang Dances with leukocytes: how tetraspanin-enriched microdomains assemble to form endothelial adhesive platforms J. Cell Biol., November 3, 2008; 183(3): 375 - 376. [Abstract] [Full Text] [PDF] A. G. Taranova, D. Maldonado III, C. M. Vachon, E. A. Jacobsen, H. Abdala-Valencia, M. P. McGarry, S. I. Ochkur, C. A. Protheroe, A. Doyle, C. S. Grant, et al. Allergic Pulmonary Inflammation Promotes the Recruitment of Circulating Tumor Cells to the Lung Cancer Res., October 15, 2008; 68(20): 8582 - 8589. [Abstract] [Full Text] [PDF] M. Yanez-Mo, O. Barreiro, P. Gonzalo, A. Batista, D. Megias, L. Genis, N. Sachs, M. Sala-Valdes, M. A. Alonso, M. C. Montoya, et al. MT1-MMP collagenolytic activity is regulated through association with tetraspanin CD151 in primary endothelial cells Blood, October 15, 2008; 112(8): 3217 - 3226. [Abstract] [Full Text] [PDF] M. Nakhaei-Nejad, A. M. Hussain, Q.-X. Zhang, and A. G. Murray Endothelial PI 3-kinase activity regulates lymphocyte diapedesis Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3608 - H3616. [Abstract] [Full Text] [PDF] J. D. van Buul, E. Kanters, and P. L. Hordijk Endothelial Signaling by Ig-Like Cell Adhesion Molecules Arterioscler Thromb Vasc Biol, September 1, 2007; 27(9): 1870 - 1876. [Abstract] [Full Text] [PDF] R. S. Pero, M. T. Borchers, K. Spicher, S. I. Ochkur, L. Sikora, S. P. Rao, H. Abdala-Valencia, K. R. O'Neill, H. Shen, M. P. McGarry, et al. G{alpha}i2-mediated signaling events in the endothelium are involved in controlling leukocyte extravasation PNAS, March 13, 2007; 104(11): 4371 - 4376. [Abstract] [Full Text] [PDF] M. Gordon-Alonso, M. Yanez-Mo, O. Barreiro, S. Alvarez, M. A. Munoz-Fernandez, A. Valenzuela-Fernandez, and F. Sanchez-Madrid Tetraspanins CD9 and CD81 Modulate HIV-1-Induced Membrane Fusion J. Immunol., October 15, 2006; 177(8): 5129 - 5137. [Abstract] [Full Text] [PDF] J. R. Cabrero, J. M. Serrador, O. Barreiro, M. Mittelbrunn, S. Naranjo-Suarez, N. Martin-Cofreces, M. Vicente-Manzanares, R. Mazitschek, J. E. Bradner, J. Avila, et al. Lymphocyte Chemotaxis Is Regulated by Histone Deacetylase 6, Independently of Its Deacetylase Activity Mol. Biol. Cell, August 1, 2006; 17(8): 3435 - 3445. [Abstract] [Full Text] [PDF] M. Sala-Valdes, A. Ursa, S. Charrin, E. Rubinstein, M. E. Hemler, F. Sanchez-Madrid, and M. Yanez-Mo EWI-2 and EWI-F Link the Tetraspanin Web to the Actin Cytoskeleton through Their Direct Association with Ezrin-Radixin-Moesin Proteins J. Biol. Chem., July 14, 2006; 281(28): 19665 - 19675. [Abstract] [Full Text] [PDF] N. Doulet, E. Donnadieu, M.-P. Laran-Chich, F. Niedergang, X. Nassif, P. O. Couraud, and S. Bourdoulous Neisseria meningitidis infection of human endothelial cells interferes with leukocyte transmigration by preventing the formation of endothelial docking structures J. Cell Biol., May 22, 2006; 173(4): 627 - 637. [Abstract] [Full Text] [PDF]

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