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Blood, 15 July 2005, Vol. 106, No. 2, pp. 531-533. Prepublished online as a Blood First Edition Paper on April 5, 2005; DOI 10.1182/blood-2004-10-3996. This Article Full Text (PDF) All Versions of this Article: 2004-10-3996v1 106/2/531    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Beier, F. Articles by Brummendorf, T. H Search for Related Content PubMed PubMed Citation Articles by Beier, F. Articles by Brummendorf, T. H Related Collections Related Letter in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 15, 2004 Accepted March 6, 2005 Accelerated telomere shortening in glycosylphosphatidylinositol (GPI)-negative as compared to GPI-positive granulocytes from patients with Paroxysmal Nocturnal Hemoglobinuria (PNH) detected by Proaerolysin Flow-Fish Fabian Beier, Stefan Balabanov, Tom Buckley, Klaus Dietz, Ulrike Hartmann, Markus Rojewski, Lothar Kanz, Hubert Schrezenmeier, and Tim H Brummendorf* Division of Hematology, Oncology and Immunology, University of Tuebingen, Tuebingen, Germany Department of Biochemistry and Microbiology, University of Victoria, Victoria, B.C., Canada Department of Medical Biometry, University of Tuebingen, Tuebingen, Germany Department of Transfusion Medicine, University of Ulm and Institute for Clinical Transfusion Medicine and Immunogenetics, Ulm, Germany * Corresponding author; email: t.bruemmendorf{at}uke.uni-hamburg.de. Telomere length has been linked to disease stage and degree of (pan-)cytopenia in patients with bone marrow failure syndromes. The aim of the current study was to analyze the impact of replicative stress on telomere length in residual GPI(+) vs. GPI(-) hematopoiesis in patients with PNH. Peripheral blood granulocytes from 16 patients and 22 healthy individuals were analyzed. For this purpose, we developed Proaerolysin flow-FISH, a novel methodology which combines Proaerolysin staining (for GPI expression) with flow-FISH (for telomere length measurement). We found significantly shortened telomeres in GPI(-) granulocytes (mean ± SE: 6.26 ± 0.27 telomere fluorescence units (TFU), both compared to their GPI(+) counterparts (6.88 ± 0.38 TFU; p=0.03) as well as to age-matched healthy individuals (7.73 ± 0.23 TFU; p< 0.001). Our findings are in support of a selective growth advantage model of PNH assuming that damage to the GPI(+) HSC compartment leads to compensatory hyperproliferation of residual GPI(-) HSC. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Letter in Blood Online: Units of analysis in accelerated telomere shortening in glycosylphosphatidylinositol (GPI)-negative compared with GPI-positive granulocytes from patients with paroxysmal nocturnal hemoglobinuria (PNH) detected by proaerolysin flow-FISH Azadeh Stark, Daniel Schultz, Klaus Dietz, Fabian Beier, Stefan Balabanov, Hubert Schrezenmeier, and Tim H. Brümmendorf Blood 2006 108: 3620. [Full Text] [PDF] This article has been cited by other articles: I. Spyridopoulos, J. Hoffmann, A. Aicher, T. H. Brummendorf, H. W. Doerr, A. M. Zeiher, and S. Dimmeler Accelerated Telomere Shortening in Leukocyte Subpopulations of Patients With Coronary Heart Disease: Role of Cytomegalovirus Seropositivity Circulation, October 6, 2009; 120(14): 1364 - 1372. [Abstract] [Full Text] [PDF] L. Mollica, I. Fleury, C. Belisle, S. Provost, D.-C. Roy, and L. Busque No Association Between Telomere Length and Blood Cell Counts in Elderly Individuals J Gerontol A Biol Sci Med Sci, September 1, 2009; 64A(9): 965 - 967. [Abstract] [Full Text] [PDF] I. Spyridopoulos, Y. Erben, T. H. Brummendorf, J. Haendeler, K. Dietz, F. Seeger, C. K. Kissel, H. Martin, J. Hoffmann, B. Assmus, et al. Telomere Gap Between Granulocytes and Lymphocytes Is a Determinant for Hematopoetic Progenitor Cell Impairment in Patients With Previous Myocardial Infarction Arterioscler Thromb Vasc Biol, May 1, 2008; 28(5): 968 - 974. [Abstract] [Full Text] [PDF] F. Beier, S. Balabanov, C.C. Amberger, U. Hartmann, K. Manger, K. Dietz, I. Kotter, and T.H. Brummendorf Telomere length analysis in monocytes and lymphocytes from patients with systemic lupus erythematosus using multi-color flow-FISH Lupus, December 1, 2007; 16(12): 955 - 962. [Abstract] [PDF] A. Stark, D. Schultz, K. Dietz, F. Beier, S. Balabanov, H. Schrezenmeier, and T. H. Brummendorf Units of analysis in accelerated telomere shortening in glycosylphosphatidylinositol (GPI)-negative compared with GPI-positive granulocytes from patients with paroxysmal nocturnal hemoglobinuria (PNH) detected by proaerolysin flow-FISH. Blood, November 15, 2006; 108(10): 3620 - 3620. [Full Text] [PDF]

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