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Blood, 15 June 2005, Vol. 105, No. 12, pp. 4598-4603. Prepublished online as a Blood First Edition Paper on February 17, 2005; DOI 10.1182/blood-2004-10-4065. This Article Full Text (PDF) All Versions of this Article: 2004-10-4065v1 105/12/4598    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wang, L. Articles by Bhatia, M. Search for Related Content PubMed PubMed Citation Articles by Wang, L. Articles by Bhatia, M. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 21, 2004 Accepted February 14, 2005 Human embryonic stem cells maintained in the absence of mouse embryonic fibroblasts or conditioned media are capable of hematopoietic development Lisheng Wang, Li Li, Pablo Menendez, Chantal Cerdan, and Mickie Bhatia* Krembil Center for Stem Cell Biology and Regenerative Medicine, Robarts Research Institute, London, ON, Canada Krembil Center for Stem Cell Biology and Regenerative Medicine, Robarts Research Institute, London, ON, Canada; Department of Microbiology and Immunology, The University of Western Ontario, London, ON, Canada * Corresponding author; email: mbhatia{at}robarts.ca. To date, hematopoietic development of human embryonic stem cells (hESCs) has been limited to cell lines cultured in the presence of either mouse embryonic fibroblasts (MEF) or MEF-conditioned media (MEF-CM). Anonymous xeno-factors from MEFs or MEF-CM complicate studies of hESC self-renewal, and also raise concerns for the potential clinical applications of generating primitive hematopoietic cells from hESC lines maintained under these ambiguous conditions. Here, we demonstrate that hESCs can be cultured over 30 passages in defined conditions in the absence of MEF or MEF-CM using only serum replacement (SR) media and high concentrations of bFGF (SR-bFGF). Similar to hESCs cultured in MEF-CM, hESCs cultured in SR-bFGF sustained characteristics of undifferentiated hESCs, proliferative potential, normal karyotype, in vitro and in vivo three germ layer specification, and gave rise to hemogenic-endothelial precursors required for subsequent primitive hematopoietic development. Our report demonstrates that anonymous factors produced by feeder cells are not necessary for hESC maintenance and subsequent hematopoietic specification, thereby providing a defined system for studies of hESC self renewal and hESC-derived hematopoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Simplifying hESC culture George Q. Daley Blood 2005 105: 4550. [Full Text] [PDF] This article has been cited by other articles: C. Unger, S. Gao, M. Cohen, M. Jaconi, R. Bergstrom, F. Holm, A. Galan, E. Sanchez, O. Irion, J. B. Dubuisson, et al. Immortalized human skin fibroblast feeder cells support growth and maintenance of both human embryonic and induced pluripotent stem cells Hum. Reprod., October 1, 2009; 24(10): 2567 - 2581. [Abstract] [Full Text] [PDF] S. C. Bendall, C. Hughes, J. L. Campbell, M. H. Stewart, P. Pittock, S. Liu, E. Bonneil, P. Thibault, M. Bhatia, and G. A. Lajoie An Enhanced Mass Spectrometry Approach Reveals Human Embryonic Stem Cell Growth Factors in Culture Mol. Cell. Proteomics, March 1, 2009; 8(3): 421 - 432. [Abstract] [Full Text] [PDF] Z. Su, C. Frye, K.-M. Bae, V. Kelley, and J. Vieweg Differentiation of Human Embryonic Stem Cells into Immunostimulatory Dendritic Cells under Feeder-Free Culture Conditions Clin. Cancer Res., October 1, 2008; 14(19): 6207 - 6217. [Abstract] [Full Text] [PDF] C. H. Martin, P. S. Woll, Z. Ni, J. C. Zuniga-Pflucker, and D. S. Kaufman Differences in lymphocyte developmental potential between human embryonic stem cell and umbilical cord blood-derived hematopoietic progenitor cells Blood, October 1, 2008; 112(7): 2730 - 2737. [Abstract] [Full Text] [PDF] S. C. Bendall, C. Hughes, M. H. Stewart, B. Doble, M. Bhatia, and G. A. Lajoie Prevention of Amino Acid Conversion in SILAC Experiments with Embryonic Stem Cells Mol. Cell. Proteomics, September 1, 2008; 7(9): 1587 - 1597. [Abstract] [Full Text] [PDF] M. Bhatia Hematopoietic Development from Human Embryonic Stem Cells Hematology, January 1, 2007; 2007(1): 11 - 16. [Abstract] [Full Text] [PDF] H. Skottman and O. Hovatta Culture conditions for human embryonic stem cells. Reproduction, November 1, 2006; 132(5): 691 - 698. [Abstract] [Full Text] [PDF] S. Mishra, B. Zhang, J. M. Cunnick, N. Heisterkamp, and J. Groffen Resistance to imatinib of bcr/abl p190 lymphoblastic leukemia cells. Cancer Res., May 15, 2006; 66(10): 5387 - 5393. [Abstract] [Full Text] [PDF] J. Lu, R. Hou, C. J. Booth, S.-H. Yang, and M. Snyder Defined culture conditions of human embryonic stem cells PNAS, April 11, 2006; 103(15): 5688 - 5693. [Abstract] [Full Text] [PDF] M. W. Lensch and G. Q. Daley Scientific and clinical opportunities for modeling blood disorders with embryonic stem cells Blood, April 1, 2006; 107(7): 2605 - 2612. [Abstract] [Full Text] [PDF] A. L. Olsen, D. L. Stachura, and M. J. Weiss Designer blood: creating hematopoietic lineages from embryonic stem cells Blood, February 15, 2006; 107(4): 1265 - 1275. [Abstract] [Full Text] [PDF] L. Vallier, M. Alexander, and R. A. Pedersen Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells J. Cell Sci., October 1, 2005; 118(19): 4495 - 4509. [Abstract] [Full Text] [PDF]

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