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Blood, 15 June 2005, Vol. 105, No. 12, pp. 4590-4597. Prepublished online as a Blood First Edition Paper on February 22, 2005; DOI 10.1182/blood-2004-10-4137. This Article Full Text (PDF) All Versions of this Article: 2004-10-4137v1 105/12/4590    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kubo, A. Articles by Keller, G. Search for Related Content PubMed PubMed Citation Articles by Kubo, A. Articles by Keller, G. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 28, 2004 Accepted February 8, 2005 The homeobox gene HEX regulates proliferation and differentiation of hemangioblasts and endothelial cells during ES cell differentiation Atsushi Kubo, Vincent Chen, Marion Kennedy, Elizabeth Zahradka, George Q Daley, and Gordon Keller* The Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY, USA Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts, USA * Corresponding author; email: gordon.keller{at}mssm.edu. In this report we have investigated the role of the homeobox gene Hex in the development and differentiation of the blast colony-forming cell (BL-CFC), a progenitor with hemangioblast characteristics generated in ES cell-derived embryoid bodies (EBs). Molecular analysis showed that Hex is expressed in mesoderm, in populations that contain BL-CFC and in blast cell colonies, the progeny of the BL-CFC. Hex-/- EBs displayed a defect in macrophage development, but generated higher numbers of BL-CFC than wild type EBs. In addition to differences in these progenitor populations, we also found that endothelial cells from the Hex-/- EBs showed enhanced proliferative potential compared to those from wild type EBs. Forced expression of Hex at the onset of ES cell differentiation resulted in reduced EB cellularity, Flk-1 expression and BL-CFC development. Taken together, these findings demonstrate that Hex functions at multiple stages of development within the differentiating EBs and uncover a novel role for this transcription factor as a negative regulator of the hemangioblast and the endothelial lineage. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Z. Huang, L. C. Dore, Z. Li, S. H. Orkin, G. Feng, S. Lin, and J. D. Crispino GATA-2 Reinforces Megakaryocyte Development in the Absence of GATA-1 Mol. Cell. Biol., September 15, 2009; 29(18): 5168 - 5180. [Abstract] [Full Text] [PDF] D. Jankovic, P. Gorello, T. Liu, S. Ehret, R. La Starza, C. Desjobert, F. Baty, M. Brutsche, P.-S. Jayaraman, A. Santoro, et al. Leukemogenic mechanisms and targets of a NUP98/HHEX fusion in acute myeloid leukemia Blood, June 15, 2008; 111(12): 5672 - 5682. [Abstract] [Full Text] [PDF] S. Pearson, P. Sroczynska, G. Lacaud, and V. Kouskoff The stepwise specification of embryonic stem cells to hematopoietic fate is driven by sequential exposure to Bmp4, activin A, bFGF and VEGF Development, April 15, 2008; 135(8): 1525 - 1535. [Abstract] [Full Text] [PDF] C. Wang, P. W. Faloon, Z. Tan, Y. Lv, P. Zhang, Y. Ge, H. Deng, and J.-W. Xiong Mouse lysocardiolipin acyltransferase controls the development of hematopoietic and endothelial lineages during in vitro embryonic stem-cell differentiation Blood, November 15, 2007; 110(10): 3601 - 3609. [Abstract] [Full Text] [PDF] R. C. R. Perlingeiro Endoglin is required for hemangioblast and early hematopoietic development Development, August 15, 2007; 134(16): 3041 - 3048. [Abstract] [Full Text] [PDF]

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