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Blood, 15 May 2005, Vol. 105, No. 10, pp. 3793-3801. Prepublished online as a Blood First Edition Paper on January 27, 2005; DOI 10.1182/blood-2004-11-4349. This Article Full Text (PDF) All Versions of this Article: 2004-11-4349v1 105/10/3793    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kortesidis, A. Articles by Gronthos, S. Search for Related Content PubMed PubMed Citation Articles by Kortesidis, A. Articles by Gronthos, S. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 15, 2004 Accepted January 13, 2005 Stromal derived factor-1 promotes the growth, survival and development of human bone marrow stromal stem cells Angela Kortesidis, Andrew Zannettino, Sandra Isenmann, Songtao Shi, Tsvee Lapidot, and Stan Gronthos* Mesenchymal Stem Cell Group, Division of Haematology, Institute of Medical and Veterinary Science/Hanson Institute, Adelaide, South Australia, Australia Myeloma and Mesenchymal Research Group, Matthew Roberts Foundation Laboratory, Division of Haematology, Institute of Medical and Veterinary Science/Hanson Institute, Adelaide, South Australia, Australia Craniofacial & Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA Department of Immunology, The Wiezmann Institute of Science, Rehovot, Israel * Corresponding author; email: stan.gronthos{at}imvs.sa.gov.au. The maintenance of bone marrow stromal stem cells (BMSSCs) is tightly controlled by the local microenvironment and by autocrine regulatory factors secreted by BMSSC. To identify such factors, a cDNA subtraction library was generated from purified BMSSC, based on their high expression of the STRO-1 antigen. Stromal derived factor-1 (SDF-1) was one differentially expressed gene highly expressed by purified BMSSC prior to culture. In vitro, immature pre-osteogenic cells expressed greater levels of SDF-1 when compared with mature cell types representative of osteoblasts and osteocytes/bone lining cells. Furthermore, SDF-1 expression was rapidly down-regulated when BMSSC were cultured under osteoinductive conditions. BMSSC were also shown to express functional cell surface SDF-1 receptors (CXCR4). Transduced BMSSC lines, secreting high SDF-1 levels, displayed an enhanced ability to form ectopic bone in vivo, in comparison with control BMSSC lines. Moreover, high SDF-1 expressing BMSSC displayed an increased capacity for cellular growth and protection against interleukin-4 induced apoptosis. Similarly, colony-forming unit-fibroblasts (CFU-F) also displayed increased growth and resistance to alpha-interferon-2a induced apoptosis, in synergy with PDGF-BB and SDF-1 in vitro. These studies indicate that the chemokine, SDF-1 may play a role in the maintenance, survival and osteogenic capacity of immature BMSSC populations. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Dominici, V. Rasini, R. Bussolari, X. Chen, T. J. Hofmann, C. Spano, D. Bernabei, E. Veronesi, F. Bertoni, P. Paolucci, et al. Restoration and reversible expansion of the osteoblastic hematopoietic stem cell niche after marrow radioablation Blood, September 10, 2009; 114(11): 2333 - 2343. [Abstract] [Full Text] [PDF] G.T.-J. Huang, S. Gronthos, and S. 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