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Blood, 1 February 2006, Vol. 107, No. 3, pp. 940-946. Prepublished online as a Blood First Edition Paper on October 4, 2005; DOI 10.1182/blood-2004-11-4403. This Article Full Text (PDF) All Versions of this Article: 2004-11-4403v1 107/3/940    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Glod, J. Articles by Fine, H. A Search for Related Content PubMed PubMed Citation Articles by Glod, J. Articles by Fine, H. A Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 2, 2004 Accepted August 25, 2005 Monocytes form a vascular barrier and participate in vessel repair after brain injury John Glod, David Kobiler, Martha Noel, Rajeth Koneru, Shoshana Lehrer, Daniel Medina, Dragan Maric, and Howard A Fine* Neuro-Oncology Branch, National Cancer Institute, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA Department of Pediatrics, UMDNJ/Robert Wood Johnson Medical School, The Cancer Institute of New Jersey, New Brunswick, NJ, USA Department of Medicine, UMDNJ/Robert Wood Johnson Medical School, The Cancer Institute of New Jersey, New Brunswick, NJ, Israel Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA * Corresponding author; email: hfine{at}mail.nih.gov. Subpopulations of bone marrow-derived cells can be induced to assume a number of endothelial properties in vitro. However, their ability to form a functional vascular barrier has not been demonstrated. We report that human CD14+ peripheral blood monocytes cultured under angiogenic conditions develop a number of phenotypic and functional properties similar to brain microvascular endothelial cells. These cells express the tight junction proteins zonula occludens 1 (ZO-1) and occludin and form a barrier with a trans-cellular electrical resistance (TCER) greater than 100 ohm cm2 and low permeability to 4 kD and 20 kD dextrans. The TCER of the cellular barrier is decreased by bradykinin and histamine. We also demonstrate that these cells are associated with repairing vasculature in areas of brain and skin injury. Our data suggest that CD14+ peripheral blood monocytes participate in the repair of the vascular barrier after brain injury. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. C. Dominguez-Punaro, M. Segura, M.-M. Plante, S. Lacouture, S. Rivest, and M. Gottschalk Streptococcus suis Serotype 2, an Important Swine and Human Pathogen, Induces Strong Systemic and Cerebral Inflammatory Responses in a Mouse Model of Infection J. Immunol., August 1, 2007; 179(3): 1842 - 1854. [Abstract] [Full Text] [PDF] H. Murata, A. Janin, C. Leboeuf, J. Soulier, E. Gluckman, V. Meignin, and G. Socie Donor-derived cells and human graft-versus-host disease of the skin Blood, March 15, 2007; 109(6): 2663 - 2665. [Abstract] [Full Text] [PDF]

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