SEARCH:   Advanced

Blood, 15 May 2005, Vol. 105, No. 10, pp. 3871-3874. Prepublished online as a Blood First Edition Paper on January 27, 2005; DOI 10.1182/blood-2004-11-4467. This Article Full Text (PDF) All Versions of this Article: 2004-11-4467v1 105/10/3871    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Schlaeger, T. M Articles by Orkin, S. H Search for Related Content PubMed PubMed Citation Articles by Schlaeger, T. M Articles by Orkin, S. H Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 1, 2004 Accepted January 25, 2005 Tie2Cre mediated gene ablation defines the Stem Cell Leukemia gene (SCL/tal1)-dependent window during hematopoietic stem cell development Thorsten M Schlaeger, Hanna K Mikkola, Christos Gekas, Hildur B Helgadottir, and Stuart H Orkin* Division of Hematology-Oncology, Dana-Farber Cancer Institute and Children's Hospital, Harvard Medical School, Boston, MA, USA Division of Hematology-Oncology, Dana-Farber Cancer Institute and Children's Hospital, Harvard Medical School, Boston, MA, USA; Howard Hughes Medical Institute, Boston, MA, USA * Corresponding author; email: stuart_orkin{at}dfci.harvard.edu. The Stem Cell Leukemia gene (SCL/tal1) is essential for the formation of all blood lineages. SCL is first expressed in mesodermal cells that give rise to embryonic blood cells, and continues to be expressed in fetal and adult hematopoietic stem cells (HSCs). However, SCL is not required for the maintenance of established long-term repopulating (LTR) HSCs in the adult. The time point at which HSC development becomes SCL-independent has not been defined. Tie2 expression appears in hemogenic and vasculogenic sites shortly after SCL. We therefore used the Tie2Cre mouse to inactivate SCL early during embryonic and fetal hematopoiesis. Tie2Cre completely inactivated SCL in yolk sac, AGM (aorta-gonad-mesonephros-region) and fetal liver hematopoietic cells and circulating blood cells. However, the fetal liver was colonized by functional LTR-HSCs. Yet, SCL remained crucial for proper differentiation of both primitive and definitive red cells and megakaryocytes. These results indicate that the SCL-dependent phase of HSC development ends before Tie2Cre mediated gene ablation becomes effective. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: A tale of two lineages: the origins of blood and endothelium Anthony R. Green Blood 2005 105: 3758. [Full Text] [PDF] This article has been cited by other articles: C. Gekas, K. E. Rhodes, L. M. Gereige, H. Helgadottir, R. Ferrari, S. K. Kurdistani, E. Montecino-Rodriguez, R. Bassel-Duby, E. Olson, A. V. Krivtsov, et al. Mef2C is a lineage-restricted target of Scl/Tal1 and regulates megakaryopoiesis and B-cell homeostasis Blood, April 9, 2009; 113(15): 3461 - 3471. [Abstract] [Full Text] [PDF] M. T. Kassouf, H. Chagraoui, P. Vyas, and C. Porcher Differential use of SCL/TAL-1 DNA-binding domain in developmental hematopoiesis Blood, August 15, 2008; 112(4): 1056 - 1067. [Abstract] [Full Text] [PDF] M.-J. Yoon, B.-K. Koo, R. Song, H.-W. Jeong, J. Shin, Y.-W. Kim, Y.-Y. Kong, and P.-G. Suh Mind bomb-1 Is Essential for Intraembryonic Hematopoiesis in the Aortic Endothelium and the Subaortic Patches Mol. Cell. Biol., August 1, 2008; 28(15): 4794 - 4804. [Abstract] [Full Text] [PDF] B. Bolinger, P. Krebs, Y. Tian, D. Engeler, E. Scandella, S. Miller, D. C. Palmer, N. P. Restifo, P.-A. Clavien, and B. Ludewig Immunologic ignorance of vascular endothelial cells expressing minor histocompatibility antigen Blood, May 1, 2008; 111(9): 4588 - 4595. [Abstract] [Full Text] [PDF] S. Ogilvy, R. Ferreira, S. G. Piltz, J. M. Bowen, B. Gottgens, and A. R. Green The SCL +40 Enhancer Targets the Midbrain Together with Primitive and Definitive Hematopoiesis and Is Regulated by SCL and GATA Proteins Mol. Cell. Biol., October 15, 2007; 27(20): 7206 - 7219. [Abstract] [Full Text] [PDF] S. S. Perry, Y. Zhao, L. Nie, S. W. Cochrane, Z. Huang, and X.-H. Sun Id1, but not Id3, directs long-term repopulating hematopoietic stem-cell maintenance Blood, October 1, 2007; 110(7): 2351 - 2360. [Abstract] [Full Text] [PDF] X.-S. Liu, X.-H. Li, Y. Wang, R.-Z. Shu, L. Wang, S.-Y. Lu, H. Kong, Y.-E Jin, L.-J. Zhang, J. Fei, et al. Disruption of palladin leads to defects in definitive erythropoiesis by interfering with erythroblastic island formation in mouse fetal liver Blood, August 1, 2007; 110(3): 870 - 876. [Abstract] [Full Text] [PDF] Z. Li, M. J. Chen, T. Stacy, and N. A. Speck Runx1 function in hematopoiesis is required in cells that express Tek Blood, January 1, 2006; 107(1): 106 - 110. [Abstract] [Full Text] [PDF]

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020