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Blood, 1 July 2005, Vol. 106, No. 1, pp. 224-226. Prepublished online as a Blood First Edition Paper on March 10, 2005; DOI 10.1182/blood-2004-11-4514. This Article Full Text (PDF) All Versions of this Article: 2004-11-4514v1 106/1/224    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ungureanu, D. Articles by Silvennoinen, O. Search for Related Content PubMed PubMed Citation Articles by Ungureanu, D. Articles by Silvennoinen, O. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 30, 2004 Accepted February 24, 2005 SUMO-1 conjugation selectively modulates STAT1-mediated gene responses Daniela Ungureanu, Sari Vanhatupa, Juha Gronholm, Jorma J Palvimo, and Olli Silvennoinen* Institute of Medical Technology, University of Tampere, Tampere, Finland Department of Clinical Microbiology, Tampere University Hospital, Tampere, Finland Institute of Medical Technology, University of Tampere, Tampere, Finland; Department of Medical Biochemistry, University of Kuopio, Kuopio, Finland * Corresponding author; email: olli.silvennoinen{at}uta.fi. STAT1 is a critical mediator of interferon (IFN)-induced gene responses. Recently, STAT1 was found to become modified by SUMO-1 conjugation at Lys703 through the SUMO E3 ligase function of PIAS proteins. However, the physiological function of sumoylation in STAT1 is still unclear. Here, we show that mutations in SUMO attachment site in STAT1 result in increased transcriptional activity in a fashion that is selective among IFN-gamma target genes. Sumoylation defective STAT1 mutant displayed increased induction of GBP1 and TAP1 transcription but not IRF-1 transcription. Moreover, the sumoylation defective mutant STAT1-KR showed a prolonged DNA-binding activity and nuclear localisation in response to IFN-gamma stimulation. These results suggest that sumoylation has a defined negative regulatory effect on selective STAT1-mediated transcription responses. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Benekli, H. Baumann, and M. Wetzler Targeting Signal Transducer and Activator of Transcription Signaling Pathway in Leukemias J. Clin. Oncol., September 10, 2009; 27(26): 4422 - 4432. [Abstract] [Full Text] [PDF] S. Weber, F. Maass, M. Schuemann, E. Krause, G. Suske, and U.-M. Bauer PRMT1-mediated arginine methylation of PIAS1 regulates STAT1 signaling Genes & Dev., January 1, 2009; 23(1): 118 - 132. [Abstract] [Full Text] [PDF] L. Song, S. Bhattacharya, A. A. Yunus, C. D. Lima, and C. Schindler Stat1 and SUMO modification Blood, November 15, 2006; 108(10): 3237 - 3244. [Abstract] [Full Text] [PDF]

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