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Blood, 15 July 2005, Vol. 106, No. 2, pp. 534-541. Prepublished online as a Blood First Edition Paper on March 31, 2005; DOI 10.1182/blood-2004-12-4653. This Article Full Text (PDF) Supplemental Tables All Versions of this Article: 2004-12-4653v1 106/2/534    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sumanas, S. Articles by Lin, S. Search for Related Content PubMed PubMed Citation Articles by Sumanas, S. Articles by Lin, S. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 6, 2004 Accepted March 22, 2005 Identification of novel vascular endothelial-specific genes by the microarray analysis of the zebrafish cloche mutants Saulius Sumanas, Tatyana Jorniak, and Shuo Lin* Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA * Corresponding author; email: shuolin{at}ucla.edu. The zebrafish cloche (clo) mutation affects the earliest known step in differentiation of blood and endothelial cells in vertebrates. We established clo/gata1-GFP transgenic line with erythroid-specific GFP expression which allowed differentiation of clo and wild type siblings at the mid-somitogenesis stages before morphologically visible phenotypes appeared. To discover novel genes potentially involved in hematopoietic and vascular development, we performed microarray analysis of over 15,000 zebrafish genes or expressed sequence tags (ESTs) in clo mutant embryos. We isolated the full-length sequences and determined the expression patterns for eight novel cDNAs which were significantly downregulated in clo-/- embryos. Dual specificity phosphatase 5 (dusp5), cadherin 5 (cdh5; VE-cadherin), aquaporin 8 (aqp8), adrenomedullin receptor (admr), complement receptor C1qR-like (crl), scavenger receptor class F, member 1 (scarf1) and ETS1-like protein (etsrp) were specifically expressed in the vascular endothelial cells while retinol binding protein 4 (rbp4) was expressed in the yolk syncytial layer and the hypochord. Further functional studies of these novel genes should help to elucidate critical early steps leading to the formation of vertebrate blood vessels. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Nicoli, C. Tobia, L. Gualandi, G. De Sena, and M. Presta Calcitonin receptor-like receptor guides arterial differentiation in zebrafish Blood, May 15, 2008; 111(10): 4965 - 4972. [Abstract] [Full Text] [PDF] J.-W. Xiong, Q. Yu, J. Zhang, and J. D. Mably An Acyltransferase Controls the Generation of Hematopoietic and Endothelial Lineages in Zebrafish Circ. Res., May 9, 2008; 102(9): 1057 - 1064. [Abstract] [Full Text] [PDF] S. Sumanas, G. Gomez, Y. Zhao, C. Park, K. Choi, and S. Lin Interplay among Etsrp/ER71, Scl, and Alk8 signaling controls endothelial and myeloid cell formation Blood, May 1, 2008; 111(9): 4500 - 4510. [Abstract] [Full Text] [PDF] S. Cermenati, S. Moleri, S. Cimbro, P. Corti, L. Del Giacco, R. Amodeo, E. Dejana, P. Koopman, F. Cotelli, and M. Beltrame Sox18 and Sox7 play redundant roles in vascular development Blood, March 1, 2008; 111(5): 2657 - 2666. [Abstract] [Full Text] [PDF] V. Ramachandran, T. Arumugam, R. F. Hwang, J. K. Greenson, D. M. Simeone, and C. D. Logsdon Adrenomedullin Is Expressed in Pancreatic Cancer and Stimulates Cell Proliferation and Invasion in an Autocrine Manner via the Adrenomedullin Receptor, ADMR Cancer Res., March 15, 2007; 67(6): 2666 - 2675. [Abstract] [Full Text] [PDF] G. Hu, J. Tang, B. Zhang, Y. Lin, J.-i. Hanai, J. Galloway, V. Bedell, N. Bahary, Z. Han, R. Ramchandran, et al. A novel endothelial-specific heat shock protein HspA12B is required in both zebrafish development and endothelial functions in vitro J. Cell Sci., October 1, 2006; 119(19): 4117 - 4126. [Abstract] [Full Text] [PDF] K. Goishi, A. Shimizu, G. Najarro, S. Watanabe, R. Rogers, L. I. Zon, and M. Klagsbrun {alpha}A-crystallin expression prevents {gamma}-crystallin insolubility and cataract formation in the zebrafish cloche mutant lens Development, July 1, 2006; 133(13): 2585 - 2593. [Abstract] [Full Text] [PDF]

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