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Blood, 15 August 2005, Vol. 106, No. 4, pp. 1215-1222. Prepublished online as a Blood First Edition Paper on April 28, 2005; DOI 10.1182/blood-2004-12-4670. This Article Full Text (PDF) All Versions of this Article: 2004-12-4670v1 106/4/1215    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mandle, T. Articles by Kempf, V. A Search for Related Content PubMed PubMed Citation Articles by Mandle, T. Articles by Kempf, V. A Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 7, 2004 Accepted April 10, 2005 Infection of human CD34+ progenitor cells with Bartonella henselae results in intraerythrocytic presence of B. henselae Tanja Mandle, Hermann Einsele, Martin Schaller, Diana Neumann, Wichard Vogel, Ingo B Autenrieth, and Volkhard A Kempf* Institut fur Medizinische Mikrobiologie und Hygiene, Eberhard-Karls-Universitat Tubingen, Tubingen, Germany Medizinische Universitatsklinik II, Eberhard-Karls-Universitat Tubingen, Tubingen, Germany Universitats-Hautklinik, Eberhard-Karls-Universitat Tubingen, Tubingen, Germany * Corresponding author; email: volkhard.kempf{at}med.uni-tuebingen.de. Although there is evidence that endothelial cells are important targets for human pathogenic Bartonella spp., the primary niche of infection is unknown. Here we elucidated whether human CD34+ hematopoietic progenitor cells (HPCs) internalize B. henselae and may serve as a potential niche of the pathogen. We showed that B. henselae does not adhere to or invade human erythrocytes. In contrast, B. henselae invades and persists in HPCs as shown by gentamicin protection assays, confocal laser scanning microscopy (CLSM) and electron microscopy (EM). FACS analysis of glycophorin A expression revealed that erythroid differentiation of HPCs was unaffected following infection with B. henselae. The number of intracellular B. henselae continuously increased over a 13-day period. When HPCs were infected with B. henselae immediately after isolation, intracellular bacteria were subsequently detectable in differentiated erythroid cells on day 9 and 13 post infection, as shown by CLSM, EM and FACS analysis. Our data provide for the first time evidence that a bacterial pathogen is able to infect and persist in differentiating HPCs, and suggests that HPCs might serve as a potential primary niche in Bartonella infections. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. B. Breitschwerdt, R. G. Maggi, W. L. Nicholson, N. A. Cherry, and C. W. Woods Bartonella sp. Bacteremia in Patients with Neurological and Neurocognitive Dysfunction J. Clin. Microbiol., September 1, 2008; 46(9): 2856 - 2861. [Abstract] [Full Text] [PDF] P. Salvatore, A. Casamassimi, L. Sommese, C. Fiorito, A. Ciccodicola, R. Rossiello, B. Avallone, V. Grimaldi, V. Costa, M. Rienzo, et al. Detrimental effects of Bartonella henselae are counteracted by L-arginine and nitric oxide in human endothelial progenitor cells PNAS, July 8, 2008; 105(27): 9427 - 9432. [Abstract] [Full Text] [PDF] T. A. Florin, T. E. Zaoutis, and L. B. Zaoutis Beyond Cat Scratch Disease: Widening Spectrum of Bartonella henselae Infection Pediatrics, May 1, 2008; 121(5): e1413 - e1425. [Abstract] [Full Text] [PDF] R. Ridder-Schroter, A. Marx, M. Beer, D. Tappe, H.-W. Kreth, and H. J. Girschick Abscess-forming lymphadenopathy and osteomyelitis in children with Bartonella henselae infection J. Med. Microbiol., April 1, 2008; 57(4): 519 - 524. [Abstract] [Full Text] [PDF] J. Berghoff, J. Viezens, L. Guptill, M. Fabbi, and M. Arvand Bartonella henselae exists as a mosaic of different genetic variants in the infected host Microbiology, July 1, 2007; 153(7): 2045 - 2051. [Abstract] [Full Text] [PDF]

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