SEARCH:   Advanced

Blood, 15 June 2005, Vol. 105, No. 12, pp. 4674-4676. Prepublished online as a Blood First Edition Paper on February 24, 2005; DOI 10.1182/blood-2004-12-4701. This Article Full Text (PDF) All Versions of this Article: 2004-12-4701v1 105/12/4674    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yarovoi, H. Articles by Poncz, M. Search for Related Content PubMed PubMed Citation Articles by Yarovoi, H. Articles by Poncz, M. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 13, 2004 Accepted February 21, 2005 Intracellular interaction of Von Willebrand Factor and Factor VIII depends on cellular context: lessons from Platelet Expressed Factor VIII Helen Yarovoi, Alan T Nurden, Robert R Montgomery, Paquita Nurden, and Mortimer Poncz* Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, PA, USA IFR4, Hopital Cardiologique, Pessac, France Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI, USA Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, PA, USA; Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA, USA * Corresponding author; email: poncz{at}email.chop.edu. We have previously reported that ectopically expressed Factor (F) VIII is stored within platelets and is released upon platelet activation. Studies in various cell lines by others have suggested that having Von Willebrand Factor (VWF) coexpression was necessary for FVIII granular storage and for its secretion. We tested the importance of VWF coexpression for ectopic storage of FVIII in platelets and for its bioavailability. Transgenic mice expressing platelet-specific FVIII were crossed onto a VWF-/- background. Antigenic levels of platelet FVIII in these mice were nearly unchanged whether VWF was present or not. Whole blood clotting times and FeCl3 carotid artery injury correction demonstrated that platelet FVIII demonstrably improved the bleeding diathesis in FVIIInull mice independent of the platelets' VWF status. Immunogold electron microscopy demonstrated that platelet FVIII is stored in platelet -granules independent of the presence of VWF. It appears that FVIII's interaction with VWF and its intracellular transportation, storage and secretion differ greatly depending on the cell type. The molecular basis for these differences now need to be elucidated. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. L. Haberichter VWF and FVIII: the origins of a great friendship Blood, March 26, 2009; 113(13): 2873 - 2874. [Full Text] [PDF] M. Neyman, J. Gewirtz, and M. Poncz Analysis of the spatial and temporal characteristics of platelet-delivered factor VIII-based clots Blood, August 15, 2008; 112(4): 1101 - 1108. [Abstract] [Full Text] [PDF]

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020