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Blood, 1 September 2005, Vol. 106, No. 5, pp. 1718-1725.
Prepublished online as a Blood First Edition Paper on May 17, 2005; DOI 10.1182/blood-2004-12-4762.
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Submitted December 14, 2004
Accepted May 2, 2005
Identification of NKG2A and NKp80 as specific Natural Killer cell markers in rhesus and pig-tailed monkeys
Domenico Mavilio*, Janet Benjamin, Diana Kim, Gabriella Lombardo, Marybeth Daucher, Audrey Kinter, Elizabeth Nies-Kraske, Emanuela Marcenaro, Alessandro Moretta, and Anthony S Fauci
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA; Dipartimento di Medicina Sperimentale, University of Genova, Genova, Italy
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Dipartimento di Medicina Sperimentale, University of Genova, Genova, Italy
Dipartimento di Medicina Sperimentale, University of Genova, Genova, Italy; Centro di Eccellenza per la Ricerca Biomedica, University of Genova, Genova, Italy
* Corresponding author; email: dmavilio{at}niaid.nih.gov.
Investigations of Natural Killer (NK) cells in simian models of disease have been hampered by a lack of appropriate phenotypic markers and by an inadequate understanding of the regulation of NK cell activities. In the present study, a panel of monoclonal antibodies (mAbs) specific for various human NK receptors was screened for cross-reactivity with NK cells from rhesus macaques and pig-tailed macaques. Flow cytometric analyses using anti-human NKG2A and anti-human NKp80 mAbs individually, and particularly in combination with anti-CD16 mAb, allowed for the identification of the entire NK cell population in both species. NK cells in monkeys were generally identified by negative selection of PBMCs for the absence of T cell, B cell and monocyte markers. mAb mediated ligation of NKp80 induced NK cell cytotoxicity, while in the case of NKG2A it displayed a clear capability to inhibit the lysis of target cells by NK cells from macaques, as well as from humans. This new phenotypic and functional characterization of NKG2A and NKp80 in rhesus and pig-tailed macaque NK cells provides a new approach in the analysis of their innate immune system.
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