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Blood, 1 June 2005, Vol. 105, No. 11, pp. 4272-4281. Prepublished online as a Blood First Edition Paper on February 8, 2005; DOI 10.1182/blood-2004-12-4883. This Article Full Text (PDF) All Versions of this Article: 2004-12-4883v1 105/11/4272    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Buitenhuis, M. Articles by Coffer, P. J Search for Related Content PubMed PubMed Citation Articles by Buitenhuis, M. Articles by Coffer, P. J Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 30, 2004 Accepted January 8, 2005 Differential regulation of granulopoiesis by the basic Helix Loop Helix transcriptional inhibitors Id1 and Id2 Miranda Buitenhuis, Hanneke W van Deutekom, Liesbeth P Verhagen, Anders Castor, Sten Eirik W Jacobsen, Jan-Willem J Lammers, Leo Koenderman, and Paul J Coffer* Department of Pulmonary Diseases, University Medical Center, Utrecht, The Netherlands Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund, Sweden Department of Pulmonary Diseases, University Medical Center, Utrecht, The Netherlands; Molecular Immunology Lab, Department of Immunology, University Medical Center, Utrecht, The Netherlands * Corresponding author; email: p.coffer{at}azu.nl. Id proteins function as inhibitors of members of the basic Helix-Loop-Helix family of transcription factors and have been demonstrated to play an important role in regulating lymphopoiesis. However, the role of these proteins in regulation of myelopoiesis is currently unclear. In this study, we have investigated the role of Id1 and Id2 in the regulation of granulopoiesis. Id1 expression was initially upregulated during early granulopoiesis, which was then followed by a decrease in expression during final maturation. In contrast, Id2 expression was upregulated in terminally differentiated granulocytes. In order to determine whether Id expression plays a critical role in regulating granulopoiesis, Id1 and Id2 were ectopically expressed in CD34+ cells by retroviral transduction. Our experiments demonstrate that constitutive expression of Id1 inhibits eosinophil development, whereas in contrast neutrophil differentiation was modestly enhanced. Constitutive Id2 expression accelerates final maturation of both eosinophils and neutrophils, whereas inhibition of Id2 expression blocks differentiation of both lineages. Transplantation of 2-microglobulin-/-NOD/SCID mice with CD34+ cells ectopically expressing Id1 resulted in enhanced neutrophil development, whereas ectopic expression of Id2 induced both eosinophil and neutrophil development. These data demonstrate that both Id1 and Id2 play a critical, although differential role in granulopoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. R. Geest, M. Buitenhuis, M. J. A. Groot Koerkamp, F. C. P. Holstege, E. Vellenga, and P. J. Coffer Tight control of MEK-ERK activation is essential in regulating proliferation, survival, and cytokine production of CD34+-derived neutrophil progenitors Blood, October 15, 2009; 114(16): 3402 - 3412. [Abstract] [Full Text] [PDF] H. C. Suh, M. Ji, J. Gooya, M. 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