SEARCH:   Advanced

Blood, 15 July 2005, Vol. 106, No. 2, pp. 584-592. Prepublished online as a Blood First Edition Paper on April 5, 2005; DOI 10.1182/blood-2004-12-4942. This Article Full Text (PDF) Supplemental Videos All Versions of this Article: 2004-12-4942v1 106/2/584    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yang, L. Articles by Luscinskas, F. W Search for Related Content PubMed PubMed Citation Articles by Yang, L. Articles by Luscinskas, F. W Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 7, 2005 Accepted March 15, 2005 ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF- activated vascular endothelium under flow Lin Yang, Richard M Froio, Tracey E Sciuto, Ann M Dvorak, Ronen Alon, and Francis W Luscinskas* Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA Departments of Pathology, Beth Israel-Deaconess Medical Center and Harvard Medical School, Boston, MA, USA Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel * Corresponding author; email: fluscinskas{at}rics.bwh.harvard.edu. In vivo, leukocyte transendothelial migration (TEM) occurs at endothelial cell junctions (paracellular) and non-junctional (transcellular) locations whereas in vitro models report TEM is mostly paracellular. The mechanisms that control the route of leukocyte TEM remain unknown. Here we tested the hypothesis that elevated ICAM-1 expression regulates the location of polymorphonuclear leukocyte (PMN) TEM. We used an in vitro flow model of TNF- activated human umbilical vein endothelium (HUVEC) or a HUVEC cell line transfected with ICAM-1GFP and live cell fluorescence microscopy to quantify the location of PMN adhesion and TEM. We observed robust transcellular TEM with TNF- activated HUVEC and ICAM-1GFP iHUVEC. In contrast, primary CD3+ T lymphocytes exclusively used a paracellular route. Endothelial ICAM-1 was identified as essential for both paracellular and transcellular PMN transmigration and interfering with ICAM-1 cytoplasmic tail function preferentially reduced transcellular TEM. We also found that ICAM-1 surface density and distribution as well as endothelial cell shape contributed to transcellular TEM. In summary, ICAM-1 promotes junctional and non-junctional TEM across inflamed vascular endothelium via distinct cytoplasmic tail associations. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Neutrophil migration across endothelium: transcellular or paracellular? Ke Zen and Charles A. Parkos Blood 2005 106: 394-395. [Full Text] [PDF] This article has been cited by other articles: Z. Mamdouh, A. Mikhailov, and W. A. Muller Transcellular migration of leukocytes is mediated by the endothelial lateral border recycling compartment J. Exp. Med., November 23, 2009; 206(12): 2795 - 2808. [Abstract] [Full Text] [PDF] K. Dorgham, A. Ghadiri, P. Hermand, M. Rodero, L. Poupel, M. Iga, O. Hartley, G. Gorochov, C. Combadiere, and P. Deterre An engineered CX3CR1 antagonist endowed with anti-inflammatory activity J. Leukoc. Biol., October 1, 2009; 86(4): 903 - 911. [Abstract] [Full Text] [PDF] C. V. Carman Mechanisms for transcellular diapedesis: probing and pathfinding by `invadosome-like protrusions' J. Cell Sci., September 1, 2009; 122(17): 3025 - 3035. [Abstract] [Full Text] [PDF] W. A. Muller Mechanisms of Transendothelial Migration of Leukocytes Circ. Res., July 31, 2009; 105(3): 223 - 230. [Abstract] [Full Text] [PDF] J. Keuschnigg, T. Henttinen, K. Auvinen, M. Karikoski, M. Salmi, and S. Jalkanen The prototype endothelial marker PAL-E is a leukocyte trafficking molecule Blood, July 9, 2009; 114(2): 478 - 484. [Abstract] [Full Text] [PDF] M. Phillipson, B. Heit, S. A. Parsons, B. Petri, S. C. Mullaly, P. Colarusso, R. M. Gower, G. Neely, S. I. Simon, and P. Kubes Vav1 Is Essential for Mechanotactic Crawling and Migration of Neutrophils out of the Inflamed Microvasculature J. Immunol., June 1, 2009; 182(11): 6870 - 6878. [Abstract] [Full Text] [PDF] S. Marmon, J. Hinchey, P. Oh, M. Cammer, C. J. de Almeida, L. Gunther, C. S. Raine, and M. P. Lisanti Caveolin-1 Expression Determines the Route of Neutrophil Extravasation through Skin Microvasculature Am. J. Pathol., February 1, 2009; 174(2): 684 - 692. [Abstract] [Full Text] [PDF] D G Downey, S C Bell, and J S Elborn Neutrophils in cystic fibrosis Thorax, January 1, 2009; 64(1): 81 - 88. [Abstract] [Full Text] [PDF] M. R. Sarantos, H. Zhang, U. Y. Schaff, N. Dixit, H. N. Hayenga, C. A. Lowell, and S. I. Simon Transmigration of Neutrophils across Inflamed Endothelium Is Signaled through LFA-1 and Src Family Kinase J. Immunol., December 15, 2008; 181(12): 8660 - 8669. [Abstract] [Full Text] [PDF] P. Alcaide, G. Newton, S. Auerbach, S. Sehrawat, T. N. Mayadas, D. E. Golan, P. Yacono, P. Vincent, A. Kowalczyk, and F. W. Luscinskas p120-Catenin regulates leukocyte transmigration through an effect on VE-cadherin phosphorylation Blood, October 1, 2008; 112(7): 2770 - 2779. [Abstract] [Full Text] [PDF] R. Sumagin, E. Lomakina, and I. H. Sarelius Leukocyte-endothelial cell interactions are linked to vascular permeability via ICAM-1-mediated signaling Am J Physiol Heart Circ Physiol, September 1, 2008; 295(3): H969 - H977. [Abstract] [Full Text] [PDF] M. Stefanidakis, G. Newton, W. Y. Lee, C. A. Parkos, and F. W. Luscinskas Endothelial CD47 interaction with SIRP{gamma} is required for human T-cell transendothelial migration under shear flow conditions in vitro Blood, August 15, 2008; 112(4): 1280 - 1289. [Abstract] [Full Text] [PDF] S. Mawaribuchi, K. Tamura, S. Okano, S. Takayama, Y. Yaoita, T. Shiba, N. Takamatsu, and M. Ito Tumor Necrosis Factor-{alpha} Attenuates Thyroid Hormone-Induced Apoptosis in Vascular Endothelial Cell Line XLgoo Established from Xenopus Tadpole Tails Endocrinology, July 1, 2008; 149(7): 3379 - 3389. [Abstract] [Full Text] [PDF] B. Petri, M. Phillipson, and P. Kubes The Physiology of Leukocyte Recruitment: An In Vivo Perspective J. Immunol., May 15, 2008; 180(10): 6439 - 6446. [Abstract] [Full Text] [PDF] D. R. Potter and E. R. Damiano The Hydrodynamically Relevant Endothelial Cell Glycocalyx Observed In Vivo Is Absent In Vitro Circ. Res., April 11, 2008; 102(7): 770 - 776. [Abstract] [Full Text] [PDF] M. Nakhaei-Nejad, A. M. Hussain, Q.-X. Zhang, and A. G. Murray Endothelial PI 3-kinase activity regulates lymphocyte diapedesis Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3608 - H3616. [Abstract] [Full Text] [PDF] R. Sumagin and I. H. Sarelius A role for ICAM-1 in maintenance of leukocyte-endothelial cell rolling interactions in inflamed arterioles Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2786 - H2798. [Abstract] [Full Text] [PDF] M. J. Allingham, J. D. van Buul, and K. Burridge ICAM-1-Mediated, Src- and Pyk2-Dependent Vascular Endothelial Cadherin Tyrosine Phosphorylation Is Required for Leukocyte Transendothelial Migration J. Immunol., September 15, 2007; 179(6): 4053 - 4064. [Abstract] [Full Text] [PDF] Hardev Ramandeep Singh Girn, S. Ahilathirunayagam, A. I. D. Mavor, and S. Homer-Vanniasinkam Reperfusion Syndrome: Cellular Mechanisms of Microvascular Dysfunction and Potential Therapeutic Strategies Vascular and Endovascular Surgery, September 1, 2007; 41(4): 277 - 293. [Abstract] [PDF] J. D. van Buul, E. Kanters, and P. L. Hordijk Endothelial Signaling by Ig-Like Cell Adhesion Molecules Arterioscler Thromb Vasc Biol, September 1, 2007; 27(9): 1870 - 1876. [Abstract] [Full Text] [PDF] R. M. Rao, L. Yang, G. Garcia-Cardena, and F. W. Luscinskas Endothelial-Dependent Mechanisms of Leukocyte Recruitment to the Vascular Wall Circ. Res., August 3, 2007; 101(3): 234 - 247. [Abstract] [Full Text] [PDF] R. Castriconi, A. Dondero, C. Cantoni, M. Della Chiesa, C. Prato, M. Nanni, M. Fiorini, L. Notarangelo, S. Parolini, L. Moretta, et al. Functional characterization of natural killer cells in type I leukocyte adhesion deficiency Blood, June 1, 2007; 109(11): 4873 - 4881. [Abstract] [Full Text] [PDF] M. Sircar, P. F. Bradfield, M. Aurrand-Lions, R. J. Fish, P. Alcaide, L. Yang, G. Newton, D. Lamont, S. Sehrawat, T. Mayadas, et al. Neutrophil Transmigration under Shear Flow Conditions In Vitro Is Junctional Adhesion Molecule-C Independent J. Immunol., May 1, 2007; 178(9): 5879 - 5887. [Abstract] [Full Text] [PDF] M. J. Lorenowicz, M. Fernandez-Borja, and P. L. Hordijk cAMP Signaling in Leukocyte Transendothelial Migration Arterioscler Thromb Vasc Biol, May 1, 2007; 27(5): 1014 - 1022. [Abstract] [Full Text] [PDF] K. A. Kelly, J. R. Allport, A. M. Yu, S. Sinh, E. H. Sage, R. E. Gerszten, and R. Weissleder SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration J. Leukoc. Biol., March 1, 2007; 81(3): 748 - 756. [Abstract] [Full Text] [PDF] K. Zen, C. X.-J. Chen, Y.-T. Chen, R. Wilton, and Y. Liu Receptor for Advanced Glycation Endproducts Mediates Neutrophil Migration across Intestinal Epithelium J. Immunol., February 15, 2007; 178(4): 2483 - 2490. [Abstract] [Full Text] [PDF] M. L. deSchoolmeester, H. Manku, and K. J. Else The Innate Immune Responses of Colonic Epithelial Cells to Trichuris muris Are Similar in Mouse Strains That Develop a Type 1 or Type 2 Adaptive Immune Response. Infect. Immun., November 1, 2006; 74(11): 6280 - 6286. [Abstract] [Full Text] [PDF] L. Yang, J. R. Kowalski, P. Yacono, M. Bajmoczi, S. K. Shaw, R. M. Froio, D. E. Golan, S. M. Thomas, and F. W. Luscinskas Endothelial Cell Cortactin Coordinates Intercellular Adhesion Molecule-1 Clustering and Actin Cytoskeleton Remodeling during Polymorphonuclear Leukocyte Adhesion and Transmigration J. Immunol., November 1, 2006; 177(9): 6440 - 6449. [Abstract] [Full Text] [PDF] R. Sumagin and I. H. Sarelius TNF-{alpha} activation of arterioles and venules alters distribution and levels of ICAM-1 and affects leukocyte-endothelial cell interactions Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2116 - H2125. [Abstract] [Full Text] [PDF] Z. Shulman, R. Pasvolsky, E. Woolf, V. Grabovsky, S. W. Feigelson, N. Erez, Y. Fukui, and R. Alon DOCK2 regulates chemokine-triggered lateral lymphocyte motility but not transendothelial migration Blood, October 1, 2006; 108(7): 2150 - 2158. [Abstract] [Full Text] [PDF] S. Devaraj, B. Davis, S. I. Simon, and I. Jialal CRP promotes monocyte-endothelial cell adhesion via Fc{gamma} receptors in human aortic endothelial cells under static and shear flow conditions Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1170 - H1176. [Abstract] [Full Text] [PDF] A. N. Mundhekar, D. C. Bullard, and D. F. Kucik Intracellular heterogeneity in adhesiveness of endothelium affects early steps in leukocyte adhesion Am J Physiol Cell Physiol, July 1, 2006; 291(1): C130 - C137. [Abstract] [Full Text] [PDF] Y. Persidsky, D. Heilman, J. Haorah, M. Zelivyanskaya, R. Persidsky, G. A. Weber, H. Shimokawa, K. Kaibuchi, and T. Ikezu Rho-mediated regulation of tight junctions during monocyte migration across the blood-brain barrier in HIV-1 encephalitis (HIVE) Blood, June 15, 2006; 107(12): 4770 - 4780. [Abstract] [Full Text] [PDF] N. Doulet, E. Donnadieu, M.-P. Laran-Chich, F. Niedergang, X. Nassif, P. O. Couraud, and S. Bourdoulous Neisseria meningitidis infection of human endothelial cells interferes with leukocyte transmigration by preventing the formation of endothelial docking structures J. Cell Biol., May 22, 2006; 173(4): 627 - 637. [Abstract] [Full Text] [PDF] L. Yang, J. R. Kowalski, X. Zhan, S. M. Thomas, and F. W. Luscinskas Endothelial Cell Cortactin Phosphorylation by Src Contributes to Polymorphonuclear Leukocyte Transmigration In Vitro Circ. Res., February 17, 2006; 98(3): 394 - 402. [Abstract] [Full Text] [PDF] I. H. Sarelius, J. M. Kuebel, J. Wang, and V. H. Huxley Macromolecule permeability of in situ and excised rodent skeletal muscle arterioles and venules Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H474 - H480. [Abstract] [Full Text] [PDF]

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020