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Blood, 1 November 2005, Vol. 106, No. 9, pp. 3062-3067. Prepublished online as a Blood First Edition Paper on July 14, 2005; DOI 10.1182/blood-2005-01-0146. This Article Full Text (PDF) All Versions of this Article: 2005-01-0146v1 106/9/3062    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Xue, S.-A. Articles by Stauss, H. J Search for Related Content PubMed PubMed Citation Articles by Xue, S.-A. Articles by Stauss, H. J Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 19, 2005 Accepted April 20, 2005 Elimination of human leukemia cells in NOD/SCID mice by WT1-TCR gene transduced human T cells Shao-An Xue, Liquan Gao, Daniel Hart, Roopinder Gillmore, Waseem Qasim, Adrian Thrasher, Jane Apperley, Boris Engels, Wolfgang Uckert, Emma Morris, and Hans J Stauss* Department of Immunology and Molecular Pathology, University College London, Royal Free Hospital, London, United Kingdom Molecular Immunology Unit, Institute of Child Health & Great Ormond Street Hospital, London, United Kingdom Department of Hematology, Imperial College London, London, United Kingdom Institute of Biology, Humboldt -University Berlin, Max-Delbruck-Center of Molecular Medicine, Berlin, Germany * Corresponding author; email: h.stauss{at}medsch.ucl.ac.uk. CTL specific for an HLA-A2-presented peptide epitope of the Wilms tumor antiegn-1 (WT1) can selectively kill immature human leukemia progenitor and stem cells in vitro. In this study we have used retroviral gene transfer to introduce a WT1-specific TCR into T lymphocytes obtained from leukemia patients and from healthy donors. TCR transduced T cells kill leukemia cells in vitro and display WT1-specific cytokine production. Intravenous injection of TCR transduced T cells into NOD/SCID mice harbouring human leukemia cells resulted in leukemia elimination, whilst transfer of control T cells transduced with an irrelevant TCR was ineffective. The data suggest that adoptive immunotherapy with WT1-TCR gene modified patient T cells should be considered for the treatment of leukemia. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Chaise, S. L. Buchan, J. Rice, J. Marquet, H. Rouard, M. Kuentz, G. E. Vittes, V. Molinier-Frenkel, J.-P. Farcet, H. J. Stauss, et al. DNA vaccination induces WT1-specific T-cell responses with potential clinical relevance Blood, October 1, 2008; 112(7): 2956 - 2964. [Abstract] [Full Text] [PDF] H. Conrad, K. Gebhard, H. Kronig, J. Neudorfer, D. H. Busch, C. Peschel, and H. Bernhard CTLs Directed against HER2 Specifically Cross-React with HER3 and HER4 J. Immunol., June 15, 2008; 180(12): 8135 - 8145. [Abstract] [Full Text] [PDF] A. L. Furmanski, C. Ferreira, I. Bartok, S. Dimakou, J. Rice, F. K. Stevenson, M. M. Millrain, E. Simpson, and J. Dyson Public T Cell Receptor -Chains Are Not Advantaged during Positive Selection J. Immunol., January 15, 2008; 180(2): 1029 - 1039. [Abstract] [Full Text] [PDF] S. Thomas, S.-A. Xue, M. Cesco-Gaspere, E. San Jose, D. P. Hart, V. Wong, R. Debets, B. Alarcon, E. Morris, and H. J. Stauss Targeting the Wilms Tumor Antigen 1 by TCR Gene Transfer: TCR Variants Improve Tetramer Binding but Not the Function of Gene Modified Human T Cells J. Immunol., November 1, 2007; 179(9): 5803 - 5810. [Abstract] [Full Text] [PDF] I. G. Schuster, D. H. Busch, E. Eppinger, E. Kremmer, S. Milosevic, C. Hennard, C. Kuttler, J. W. Ellwart, B. Frankenberger, E. Nossner, et al. Allorestricted T cells with specificity for the FMNL1-derived peptide PP2 have potent antitumor activity against hematologic and other malignancies Blood, October 15, 2007; 110(8): 2931 - 2939. [Abstract] [Full Text] [PDF] J. M. Goldman How I treat chronic myeloid leukemia in the imatinib era Blood, October 15, 2007; 110(8): 2828 - 2837. [Abstract] [Full Text] [PDF] J. Kuball, M. L. Dossett, M. Wolfl, W. Y. Ho, R.-H. Voss, C. Fowler, and P. D. Greenberg Facilitating matched pairing and expression of TCR chains introduced into human T cells Blood, March 15, 2007; 109(6): 2331 - 2338. [Abstract] [Full Text] [PDF] R. Gillmore, S.-A. Xue, A. Holler, J. Kaeda, D. Hadjiminas, V. Healy, R. Dina, S. C. Parry, I. Bellantuono, Y. Ghani, et al. Detection of Wilms' Tumor Antigen-Specific CTL in Tumor-Draining Lymph Nodes of Patients with Early Breast Cancer Clin. Cancer Res., January 1, 2006; 12(1): 34 - 42. [Abstract] [Full Text] [PDF]

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