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Blood, 1 July 2005, Vol. 106, No. 1, pp. 216-223. Prepublished online as a Blood First Edition Paper on March 24, 2005; DOI 10.1182/blood-2005-01-0220. This Article Full Text (PDF) Supplemental Figures and Table All Versions of this Article: 2005-01-0220v1 106/1/216    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Segura, E. Articles by Thery, C. Search for Related Content PubMed PubMed Citation Articles by Segura, E. Articles by Thery, C. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 18, 2005 Accepted March 10, 2005 ICAM-1 on exosomes from mature dendritic cells is critical for efficient naive T cell priming Elodie Segura, Carole Nicco, Berangere Lombard, Philippe Veron, Graca Raposo, Frederic Batteux, Sebastian Amigorena, and Clotilde Thery* INSERM U653, Institut Curie, Paris, France Laboratoire d'Immunologie, UPRES1833, Faculte et Hopital Cochin, Universite Paris V, Paris, France Laboratory of Mass Spectrometry and Proteomics, Institut Curie, Paris, France CNRS UMR 144, Institut Curie, Paris, France * Corresponding author; email: clotilde.thery{at}curie.fr. Exosomes are secreted vesicles formed in late endocytic compartments. Immature dendritic cells (DCs) secrete exosomes which transfer functional MHC-peptide complexes to other DCs. Since immature and mature DCs induce different functional T cell responses (i.e. tolerance versus priming), we asked whether DC maturation also influenced the priming abilities of their exosomes. We show that exosomes secreted by LPS-treated mature DCs are 50-100 fold more potent to induce antigen-specific T cell activation in vitro than exosomes from immature DCs. In vitro, exosomes from mature DCs transfer to B lymphocytes the ability to prime naive T cells. In vivo, only mature exosomes trigger effector T cell responses, leading to fast skin graft rejection. Proteomic and biochemical analyses revealed that mature exosomes are enriched in MHC class II, B7.2, ICAM-1, and bear little MFG-E8 as compared to immature exosomes. Functional analysis using DC-derived exosomes from knock-out mice showed that MHC class II and ICAM-1 are required for mature exosomes to prime naive T cells, whereas B7.2 and MFG-E8 are dispensable. Therefore, changes in protein composition and priming abilities of exosomes reflect the maturation signals received by DCs. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Lynch, S. G. Santos, E. C. Campbell, A. M. S. Nimmo, C. Botting, A. Prescott, A. N. Antoniou, and S. J. Powis Novel MHC Class I Structures on Exosomes J. Immunol., August 1, 2009; 183(3): 1884 - 1891. [Abstract] [Full Text] [PDF] M. W. Graner, O. Alzate, A. M. Dechkovskaia, J. D. Keene, J. H. Sampson, D. A. Mitchell, and D. D. Bigner Proteomic and immunologic analyses of brain tumor exosomes FASEB J, May 1, 2009; 23(5): 1541 - 1557. [Abstract] [Full Text] [PDF] Y. Qu, L. Ramachandra, S. Mohr, L. Franchi, C. V. Harding, G. Nunez, and G. R. Dubyak P2X7 Receptor-Stimulated Secretion of MHC Class II-Containing Exosomes Requires the ASC/NLRP3 Inflammasome but Is Independent of Caspase-1 J. Immunol., April 15, 2009; 182(8): 5052 - 5062. 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