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Blood, 15 September 2005, Vol. 106, No. 6, pp. 2083-2090. Prepublished online as a Blood First Edition Paper on June 2, 2005; DOI 10.1182/blood-2005-01-0283. This Article Full Text (PDF) All Versions of this Article: 2005-01-0283v1 106/6/2083    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Polli, M. Articles by Nutt, S. L Search for Related Content PubMed PubMed Citation Articles by Polli, M. Articles by Nutt, S. L Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 24, 2005 Accepted May 22, 2005 The development of functional B lymphocytes in conditional PU.1 knockout mice Matthew Polli, Aleksandar Dakic, Amanda Light, Li Wu, David M Tarlinton, and Stephen L Nutt* The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia * Corresponding author; email: nutt{at}wehi.edu.au. An abundance of research has entrenched the view that the Ets domain containing transcription factor PU.1 is fundamental to the development and function of B lymphocytes. In this study, we have made use of a conditional PU.1 allele to test this notion. Complete deletion of PU.1 resulted in the loss of B cells and all other lineage positive cells in the fetal liver and death between E18.5 and birth, however, specific deletion of PU.1 in the B lineage had no effect on B cell development. Furthermore, deletion of PU.1 in B cells did not compromise their ability to establish and maintain an immune response. An increased level of apoptosis was observed in vitro upon BCR cross-linking, however, this was partially rescued by IL-4. These findings suggest that PU.1 is not essential for the development of functional B lymphocytes beyond the preB stage. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Wang, C. H. Lee, C. Qi, P. Tailor, J. Feng, S. Abbasi, T. Atsumi, and H. C. Morse III IRF8 regulates B-cell lineage specification, commitment, and differentiation Blood, November 15, 2008; 112(10): 4028 - 4038. [Abstract] [Full Text] [PDF] C. Pridans, M. L. Holmes, M. Polli, J. M. Wettenhall, A. Dakic, L. M. Corcoran, G. K. Smyth, and S. L. Nutt Identification of Pax5 Target Genes in Early B Cell Differentiation J. Immunol., February 1, 2008; 180(3): 1719 - 1728. [Abstract] [Full Text] [PDF] R. P. DeKoter, B. L. Schweitzer, M. B. Kamath, D. Jones, H. Tagoh, C. Bonifer, D. A. Hildeman, and K. J. Huang Regulation of the Interleukin-7 Receptor {alpha} Promoter by the Ets Transcription Factors PU.1 and GA-binding Protein in Developing B Cells J. Biol. Chem., May 11, 2007; 282(19): 14194 - 14204. [Abstract] [Full Text] [PDF] W.-C. Chou, D. E. Levy, and C.-K. Lee STAT3 positively regulates an early step in B-cell development Blood, November 1, 2006; 108(9): 3005 - 3011. [Abstract] [Full Text] [PDF] B. L. Schweitzer, K. J. Huang, M. B. Kamath, A. V. Emelyanov, B. K. Birshtein, and R. P. DeKoter Spi-C Has Opposing Effects to PU.1 on Gene Expression in Progenitor B Cells J. Immunol., August 15, 2006; 177(4): 2195 - 2207. [Abstract] [Full Text] [PDF] W. Xu, T. Fukuyama, P. A. Ney, D. Wang, J. Rehg, K. Boyd, J. M. A. van Deursen, and P. K. Brindle Global transcriptional coactivators CREB-binding protein and p300 are highly essential collectively but not individually in peripheral B cells Blood, June 1, 2006; 107(11): 4407 - 4416. [Abstract] [Full Text] [PDF]

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