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Blood, 1 March 2006, Vol. 107, No. 5, pp. 1818-1827. Prepublished online as a Blood First Edition Paper on November 1, 2005; DOI 10.1182/blood-2005-01-0339. This Article Full Text (PDF) All Versions of this Article: 2005-01-0339v1 107/5/1818    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Van Gulck, E. R Articles by Vanham, G. Search for Related Content PubMed PubMed Citation Articles by Van Gulck, E. R Articles by Vanham, G. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 25, 2005 Accepted October 13, 2005 Efficient stimulation of HIV-1-specific T-cells using dendritic cells electroporated with mRNA encoding autologous HIV-1 Gag and Env protein Ellen R Van Gulck*, Peter Ponsaerts, Leo Heyndrickx, Katleen Vereecken, Filip Moerman, Ann De Roo, Robert Colebunders, Glenn Van den Bosch, Dirk R Van Bockstaele, Viggor F Van Tendeloo, Sabine Allard, Bernard Verrier, Concepcion Maranon, Guillaume Hoeffel, Anne Hosmalin, Zwi N Berneman, and Guido Vanham HIV and Retrovirology Research Unit, Department of Microbiology, Institute of Tropical medicine of Antwerp (ITMA), Antwerp, Belgium Laboratory of Experimental Hematology, Faculty of Medicine, University of Antwerp (UA), Antwerp University Hospital (UZA), Edegem, Antwerp, Belgium AIDS Clinic, Clinical Department, Institute of Tropical Medicine of Antwerp (ITMA), Antwerp, Belgium Laboratory of Physiology and Immunology, Brussels, Belgium CNRS-BioMerieux, FRE 2736, IFR 128, Lyon, France Antigen Presentation by Dendritic Cell Group, Departement d'Immunologie, Institut Cochin, INSERM U567, CNRS UMR 8104, Universite Paris V, Paris, France HIV and Retrovirology Research Unit, Department of Microbiology, Institute of Tropical medicine of Antwerp (ITMA), Antwerp, Belgium; Department of Biomedical Sciences, Faculty of Pharmaceutical, Veterinary and Biomedical Sciences, University of Antwerp, Antwerp, Belgium * Corresponding author; email: evangulck{at}itg.be. Infection with human immunodeficiency virus type 1 (HIV-1) is characterized by dysfunction of HIV-1-specific T-cells. In order to control the virus, antigen-loaded dendritic cells (DC) might be useful to boost and broaden HIV-specific T-cell responses. In the present study, monocyte-derived DC from non-treated HIV-1-seropositive patients were electroporated with codon-optimized ("humanized") mRNA encoding consensus HxB-2 (hHXB-2) Gag protein. These DC elicited a strong HIV-1 Gag-specific interferon (IFN)- response by an HLA-A2-restricted CD8+ T-cell line. Moreover, hHXB-2 gag mRNA-electroporated DC also triggered IFN- secretion by autologous peripheral blood mononuclear cells (PBMC), CD4+ T-cells and CD8+ T-cells from all patients tested. Next a novel strategy was developed, using autologous virus sequences. Significant specific IFN- T-cell responses were induced in all patients tested by DC electroporated with patients' autologous PCR-amplified and in vitro transcribed proviral and plasma viral mRNA, encoding either Gag or Env. The stimulatory effect was seen on PBMC, CD8+ T-cells and CD4+ T-cells, demonstrating both major histocompatibility complex (MHC) class I and MHC class II antigen presentation. Moreover, a significant interleukin (IL)-2 T-cell response was induced by DC electroporated with hHxB-2 or proviral gag mRNA. These findings open a major perspective for the development of patient-specific immunotherapy for HIV-1 disease. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. M. Melhem, S. M. Gleason, X. D. Liu, and S. M. Barratt-Boyes High-Level Antigen Expression and Sustained Antigen Presentation in Dendritic Cells Nucleofected with Wild-Type Viral mRNA but Not DNA Clin. Vaccine Immunol., September 1, 2008; 15(9): 1337 - 1344. [Abstract] [Full Text] [PDF] E. R. Van Gulck, G. Vanham, L. Heyndrickx, S. Coppens, K. Vereecken, D. Atkinson, E. Florence, I. Kint, Z. N. Berneman, and V. V. Tendeloo Efficient In Vitro Expansion of Human Immunodeficiency Virus (HIV)-Specific T-Cell Responses by gag mRNA-Electroporated Dendritic Cells from Treated and Untreated HIV Type 1-Infected Individuals J. Virol., April 1, 2008; 82(7): 3561 - 3573. [Abstract] [Full Text] [PDF] Z. N. Berneman, E. R. Van Gulck, L. Heyndrickx, P. Ponsaerts, V. F.I. Van Tendeloo, and G. Vanham Induction of Potentially Protective HIV-Specific T-Cell Responses In Vitro by Gag mRNA-Electroporated Dendritic Cells. Blood (ASH Annual Meeting Abstracts), November 16, 2006; 108(11): 1261 - 1261. [Abstract] [PDF] D. Finzi, S. F. Plaeger, and C. W. Dieffenbach Defective Virus Drives Human Immunodeficiency Virus Infection, Persistence, and Pathogenesis. Clin. Vaccine Immunol., July 1, 2006; 13(7): 715 - 721. [Full Text] [PDF]

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