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Blood, 15 August 2005, Vol. 106, No. 4, pp. 1415-1418. Prepublished online as a Blood First Edition Paper on April 21, 2005; DOI 10.1182/blood-2005-01-0413. This Article Full Text (PDF) All Versions of this Article: 2005-01-0413v1 106/4/1415    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Guo, Y. Articles by Yasukawa, M. Search for Related Content PubMed PubMed Citation Articles by Guo, Y. Articles by Yasukawa, M. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 31, 2005 Accepted April 12, 2005 Direct recognition and lysis of leukemia cells by WT1-specific CD4+ T lymphocytes in an HLA class II-restricted manner Yun Guo, Hironari Niiya, Taichi Azuma, Naoyuki Uchida, Yoshihiro Yakushijin, Ikuya Sakai, Takaaki Hato, Masuhiro Takahashi, Satoru Senju, Yasuharu Nishimura, and Masaki Yasukawa* First Department of Internal Medicine, Ehime University School of Medicine, Ehime, Japan School of Health Sciences, Faculty of Medicine, Niigata University, Niigata, Japan Department of Immunogenetics, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan * Corresponding author; email: yasukawa{at}m.ehime-u.ac.jp. WT1 has been recognized as an attractive target antigen of immunotherapy for various malignancies including leukemia. Since tumor-associated antigen-specific CD4+ T lymphocytes undoubtedly play an important role in the induction of an anti-tumor immune response, we attempted to generate WT1-specific CD4+ T lymphocytes in vitro and examined their anti-leukemia functions. A CD4+ T-cell line, designated NIK-1, which proliferated and produced Th1 cytokines specifically in response to stimulation with the WT1-derived peptide, WT1337-347 LSHLQMHSRKH, in an HLA-DP5-restriced manner was established. NIK-1 exhibited cytotoxicity against HLA-DP5-positive, WT1-expressing leukemia cells but did not lyse HLA-DP5-negative, WT1-expressing leukemia cells or HLA-DP5-positive, WT1-negative cells. NIK-1 did not inhibit colony formation by normal bone marrow cells of HLA-DP5-positive individuals. This is the first report to describe WT1-specific and HLA class II-restricted CD4+ T lymphocytes possessing direct cytotoxic activity against leukemia cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. J. May, T. Dao, J. Pinilla-Ibarz, T. Korontsvit, V. Zakhaleva, R. H. Zhang, P. Maslak, and D. A. Scheinberg Peptide Epitopes from the Wilms' Tumor 1 Oncoprotein Stimulate CD4+ and CD8+ T Cells That Recognize and Kill Human Malignant Mesothelioma Tumor Cells Clin. Cancer Res., August 1, 2007; 13(15): 4547 - 4555. [Abstract] [Full Text] [PDF] M. D. Tarantino and L. C. Weber Hematuria and Hemoglobinuria in Pediatric Patients Diagnosed with Newly Diagnosed Immune Thrombocytopenic Purpura. Blood (ASH Annual Meeting Abstracts), November 16, 2006; 108(11): 1088 - 1088. [Abstract] [PDF] A. Hombach, H. Kohler, G. Rappl, and H. Abken Human CD4+ T Cells Lyse Target Cells via Granzyme/Perforin upon Circumvention of MHC Class II Restriction by an Antibody-Like Immunoreceptor J. Immunol., October 15, 2006; 177(8): 5668 - 5675. [Abstract] [Full Text] [PDF]

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