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Blood, 1 November 2005, Vol. 106, No. 9, pp. 3012-3019. Prepublished online as a Blood First Edition Paper on July 19, 2005; DOI 10.1182/blood-2005-01-0433. This Article Full Text (PDF) All Versions of this Article: 2005-01-0433v1 106/9/3012    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Baksh, D. Articles by Zandstra, P. W Search for Related Content PubMed PubMed Citation Articles by Baksh, D. Articles by Zandstra, P. W Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 10, 2005 Accepted July 2, 2005 Soluble factor crosstalk between human bone marrow-derived hematopoietic and mesenchymal cells enhances in vitro CFU-F and CFU-O growth and reveals heterogeneity in the mesenchymal progenitor cell compartment Dolores Baksh, John E Davies, and Peter W Zandstra* Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada; Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada * Corresponding author; email: peter.zandstra{at}utoronto.ca. The homeostatic adult bone marrow (BM) is a complex tissue wherein physical and biochemical interactions serve to maintain a balance between the hematopoietic and non-hematopoietic compartments. To focus on soluble factor interactions occurring between mesenchymal and hematopoietic cells, a serum-free adhesion-independent culture system was developed which allows manipulation of the growth of both mesenchymal and hematopoietic human BM-derived progenitors, and the balance between these compartments. Factorial experiments demonstrated a role for stem cell factor (SCF) and interleukin-3 (IL3) in the concomitant growth of hematopoietic (CD45+) and non-hematopoietic (CD45-) cells, as well as their derivatives. Kinetic tracking of IL3 receptor (CD123) and SCF receptor (CD117) expression on a sorted CD45- cell population revealed the emergence of CD45-CD123+ cells capable of osteogenesis. Of the total CFU-F and CFU-O, approximately 24% of CFU-F and ~22% of CFU-O were recovered from this population. Cell sorting experiments demonstrated that the CD45+ cell population secreted soluble factors that positively impact the survival and proliferation of CFU-F and CFU-O generated from the CD45- cells. Together, our results provide insight into the intercellular cytokine network between hematopoietic and mesenchymal cells and provide a strategy to mutually culture both mesenchymal and hematopoietic cells in a defined scalable bioprocess. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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